Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)

Overview

  • Product name
    Anti-Alpha B Crystallin (phospho S59) antibody
    See all Alpha B Crystallin primary antibodies
  • Description
    Rabbit polyclonal to Alpha B Crystallin (phospho S59)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 54-64 (phospho S59).
    Sequence:

    FLRAPSWIDTG

  • Positive control
    • U373 MG cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21126175
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.
ICC/IF Use a concentration of 8 µg/ml.
IHC-P Use a concentration of 8 µg/ml.

Target

Images

  • ab5577 at 0.5µg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U373 cell lysate.

  • ab5577 staining of (top panel) human kidney tissue sections, highlighting highly localised staining in tubular epithelial cells, and (bottom panel) human ovarian carcinoma sections. Formalin-fixed paraffin embedded sections of human kidney and ovarian carcinoma tissue were incubated with ab5577 (1/1000) for 2 hours at room temperature. Antigen retrieval was performed by heat induction in citrate buffer, pH 6.

    See Abreview

References

This product has been referenced in:

See all 6 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult kidney)
Specification
adult kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney and ovarian carcinoma)
Specification
kidney and ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid 10mM pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid 10mM pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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