Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

Overview

  • Product nameAnti-alpha Defensin 1 + 2 + 3 antibody
  • Description
    Goat polyclonal to alpha Defensin 1 + 2 + 3
  • Specificityab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-DESLAPKHPGSRKN

    , corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1).

  • Positive control
    • Human spleen lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.

Target

  • RelevanceDefensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • DEF1 antibody
    • DEF3 antibody
    • DEFA1 antibody
    • DEFA2 antibody
    • DEFA3 antibody
    • Defensin alpha 1 antibody
    • Defensin alpha 2 antibody
    • Defensin alpha 3 antibody
    • HNP1 antibody
    • HNP3 antibody
    • HP1 antibody
    • HP3 antibody
    • MRS antibody
    • Neutrophil defensin 1 antibody
    • Neutrophil defensin 2 antibody
    • Neutrophil defensin 3 antibody
    see all

Anti-alpha Defensin 1 + 2 + 3 antibody images

  • Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 10 kDa


    Exposure time : 1 hour
  • ab99504 (3.8µg/ml) staining of paraffin embedded Human Thymus shows staining of selected cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

ab99504 has not yet been referenced specifically in any publications.

Product Wall



Nous n’avons jamais testé ni ab99504 ni ab62757 chez la souris en IHC-P.

J’ai fait une comparaison entre l’alpha Defensin 1 + 2 + 3 humaine et murin et il n’y qu’une homologie totale d’envi...

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Vielen Dank für Ihren Anruf und Ihr Interesse an unseren Produkten. Leider haben wir zur Zeit kein ELISA Kit in unserem Katalog gegen das NP-1/alpha defensin 1 protein. Wir haben drei Antikörper, die jedoch auch andere zyklische alpha Defensin e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"