Overview

  • Product name
    Anti-alpha Defensin 1 antibody
    See all alpha Defensin 1 primary antibodies
  • Description
    Goat polyclonal to alpha Defensin 1
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, from internal sequence amino acids of Human alpha Defensin 1.

  • Positive control
    • Human Thymus (Myeloid Cells) tissue; Human Spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
  • Sequence similarities
    Belongs to the alpha-defensin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha 1 antibody
    • DEF1 antibody
    • DEF1_HUMAN antibody
    • DEFA1 antibody
    • DEFA1B antibody
    • DEFA2 antibody
    • Defensin 1 antibody
    • Defensin antibody
    • Defensin, alpha 1 antibody
    • Defensin, alpha 1, myeloid related sequence antibody
    • Defensin, alpha 2 antibody
    • HNP-1 antibody
    • HNP-2 antibody
    • HNP1 antibody
    • HP-1 antibody
    • HP-2 antibody
    • HP1 antibody
    • HP2 antibody
    • MRS antibody
    • Myeloid related sequence antibody
    • Neutrophil defensin 1 antibody
    • Neutrophil defensin 2 antibody
    see all

Images

  • ab122884, at 3.75 µg/ml, staining alpha Defensin 1 in Formalin-fixed, Paraffin-embedded Human Thymus (Myeloid Cells) tissue by Immunohistochemistry.
  • Anti-alpha Defensin 1 antibody (ab122884) at 2 µg/ml + Human spleen lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 10 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
  • ab122884 staining alpha Degensin 1 in Mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 1% BSA) for 12 hours at 4°C. A DyLight® 550-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Bian F  et al. Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke. Stroke 45:1177-9 (2014). Read more (PubMed: 24558093) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Tissue sections (aorta)
Specification
aorta
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Oct 21 2013

The mouse and human defensin alpha 1 proteins does not have homology required for antibody cross reactivity so we can not confirm the antibody ab122884 will detect mouse protein as well. We advice customer looking for mouse specific antibody only becau...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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