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Synthetic peptide conjugated to KLH, from internal sequence amino acids of Human alpha Defensin 1.
Our Abpromise guarantee covers the use of ab122884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
ab122884 staining alpha Degensin 1 in Mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 1% BSA) for 12 hours at 4°C. A DyLight® 550-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.
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