Overview

  • Product name
    Anti-alpha Elastin antibody
    See all alpha Elastin primary antibodies
  • Description
    Guinea pig polyclonal to alpha Elastin
  • Host species
    Guinea pig
  • Specificity
    Cross-reaction with rat Elastin < 0.1%. Cross-reactivity in RIA at 1:500 dilution: Human collagen type I, II, III, IV, V <0.1 %; Human fibronectin < 0.1%.
  • Tested applications
    Suitable for: IHC-P, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Elastin from Human skin.

  • Positive control
    • Human skin tissue, Human aorta tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab166997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. After de-waxing the tissue slices treat with 0.2% hyaluronidase (approximately 300 U/mg) in TBS 15 min at 37°C. It is recommended to block non-specific binding blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. incubate primary for 60 min at RT or 2-8°C over night.
RIA 1/500.
ICC/IF 1/50.

Target

  • Function
    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificity
    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in disease
    Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1) [MIM:123700]. A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Belongs to the elastin family.
  • Post-translational
    modifications
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

References

ab166997 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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