Overview

  • Product nameAnti-Alpha Fodrin antibody
    See all Alpha Fodrin primary antibodies
  • Description
    Rabbit polyclonal to Alpha Fodrin
  • SpecificityThis antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin.
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 162 of mouse alpha-Fodrin

Properties

Applications

Our Abpromise guarantee covers the use of ab2558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml.

Target

  • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similaritiesBelongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    modifications
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody images

  • All lanes : Anti-Alpha Fodrin antibody (ab2558) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 285 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,71 kDa. We are unsure as to the identity of these extra bands.


  • Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa (possible cleavage fragment).

References for Anti-Alpha Fodrin antibody (ab2558)

This product has been referenced in:
  • Woods LT  et al. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303:C790-801 (2012). WB ; Mouse . Read more (PubMed: 22875784) »
  • Grubb DR  et al. Phospholipase C{beta}1b associates with a Shank3 complex at the cardiac sarcolemma. FASEB J 25:1040-7 (2011). WB ; Rat . Read more (PubMed: 21148417) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. I have investigated our records for ab2558 Alpha Fodrin antibody. Regarding mouse reactivity, the answer to this question on the datasheet was provided in 2006. We now have information to confirm that this antibody has bee...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293,HELA and JURKAT)
Loading amount 10 µg
Specification HEK293,HELA and JURKAT
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"