• Product nameAnti-Alpha Fodrin antibody
    See all Alpha Fodrin primary antibodies
  • Description
    Rabbit polyclonal to Alpha Fodrin
  • Specificityab75284 detects endogenous levels of fragment of activated Alpha Fodrin resulting from cleavage adjacent to Asp1185.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Alpha Fodrin.

  • Positive control
    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).



Our Abpromise guarantee covers the use of ab75284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 285 kDa.
ELISA 1/10000.


  • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similaritiesBelongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody images

  • All lanes : Anti-Alpha Fodrin antibody (ab75284) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from A549 cells treated with etoposide (25uM, 24hours)
    Lane 3 : Extracts from HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 285 kDa
    Observed band size : >117 kDa (why is the actual band size different from the predicted?)

References for Anti-Alpha Fodrin antibody (ab75284)

ab75284 has not yet been referenced specifically in any publications.

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