Overview

  • Product nameAnti-Alpha Fodrin antibody
    See all Alpha Fodrin primary antibodies
  • Description
    Rabbit polyclonal to Alpha Fodrin
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish
  • Immunogen

    A synthetic peptide corresponding to a region between residue 2422 and 2472 of human Alpha Fodrin (NP_003118.1).

  • Positive control
    • Human colon carcinoma tissue, human ovarian carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibody was affinity purified using an epitope specific to Alpha Fodrin immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.

    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • Sequence similaritiesBelongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • Post-translational
      modifications
      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Anti-Alpha Fodrin antibody images

    • IHC analysis of FFPE section of human ovarian carcinoma using ab84492 at a dilution of 1/250.
    • IHC analysis of FFPE section of human colon carcinoma using ab84492 at a dilution of 1/250.

    References for Anti-Alpha Fodrin antibody (ab84492)

    ab84492 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84492.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"