Recombinant
RabMAb

Anti-Alpha Fodrin antibody [EPR3017] (ab75755)

Overview

  • Product name
    Anti-Alpha Fodrin antibody [EPR3017]
    See all Alpha Fodrin primary antibodies
  • Description
    Rabbit monoclonal [EPR3017] to Alpha Fodrin
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Alpha Fodrin

  • Positive control
    • HeLa lystes, untreated and treated with Camptothecin; Jurkat cell lysates, untreated and treated with Staurosporine; TF-1, SH-SY5Y and human brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Alpha Fodrin antibody (HRP) [EPR3017] (ab199075)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 285 kDa (predicted molecular weight: 285 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Involvement in disease
      Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • Sequence similarities
      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • Post-translational
      modifications
      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Images

    • All lanes : Anti-Alpha Fodrin antibody [EPR3017] (ab75755) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with Camptothecin
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat treated with Staurosporine
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 285 kDa
      Additional bands at : 120 kDa (possible cleavage fragment),150 kDa (possible cleavage fragment),85 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-Alpha Fodrin antibody [EPR3017] (ab75755) at 1/2000 dilution

      Lane 1 : TF-1 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 285 kDa
      Additional bands at : 150 kDa (possible cleavage fragment).

    References

    This product has been referenced in:
    • Yan M  et al. Effect of glutamate on lysosomal membrane permeabilization in primary cultured cortical neurons. Mol Med Rep 13:2499-505 (2016). Read more (PubMed: 26821268) »
    • Sebe JY  et al. ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice. J Neurophysiol 109:429-36 (2013). WB . Read more (PubMed: 23100132) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your patience. The immunogen is located near the C-terminus. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat, Human ATL cell lines (KK1, KOB))
    Loading amount
    10 µg
    Specification
    Jurkat, Human ATL cell lines (KK1, KOB)
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 26 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (JURKAT)
    Loading amount
    10 µg
    Specification
    JURKAT
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 05 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - other (Brain synaptosomes)
    Loading amount
    20 µg
    Specification
    Brain synaptosomes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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