Anti-NEAS antibody [D8B7] (ab11755)
Key features and details
- Mouse monoclonal [D8B7] to NEAS
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG2b
Overview
-
Product name
Anti-NEAS antibody [D8B7]
See all NEAS primary antibodies -
Description
Mouse monoclonal [D8B7] to NEAS -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
Full length native protein (purified) corresponding to Human NEAS.
-
Positive control
- IHC-P: Mouse and Rat brain tissue. ICC: 3T3, HeLa cells.
-
General notes
This product was changed from ascites to tissue culture supernatant on 21/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
D8B7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
Use at an assay dependent concentration. See Abreviews.
|
ICC |
Use at an assay dependent concentration.
|
|
WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 297 kDa.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. See Abreviews. |
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 297 kDa. |
Target
-
Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
Involvement in disease
Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. -
Sequence similarities
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
Post-translational
modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
-
Database links
- Entrez Gene: 6709 Human
- Entrez Gene: 64159 Rat
- Omim: 182810 Human
- SwissProt: Q13813 Human
- SwissProt: P16546 Mouse
- SwissProt: P16086 Rat
- Unigene: 372331 Human
- Unigene: 204969 Mouse
see all -
Alternative names
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
see all
Images
-
Lanes 2-6 : Anti-NEAS antibody [D8B7] (ab11755) at 0.5 µg/ml
Lane 1 : MW marker
Lane 2 : Human Brain lysate at 20 µg
Lane 3 : Mouse Brain lysate at 20 µg
Lane 4 : Rat Brain lysate at 20 µg
Lane 5 : 3T3 cell lysate at 20 µg
Lane 6 : HeLa cell lysate at 20 µg
Secondary
Lanes 2-6 : HRP labeled goat anti-mouse IgG
Predicted band size: 297 kDa -
ICC staining of purified ab11755 on 3T3 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
-
IHC staining of purified ab11755 on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
-
Lanes 2-3 : Anti-NEAS antibody [D8B7] (ab11755) at 0.1 µg/ml
Lane 1 : MW marker
Lane 2 : Drosophila head lysate at 20 µg
Lane 3 : Drosophila S2 (embryonic) cell lysate at 20 µg
Secondary
Lanes 2-3 : HRP-labeled goat anti-mouse IgG
Predicted band size: 297 kDa -
ICC staining of purified ab11755 on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
-
IHC staining of purified ab11755 on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
-
Anti-NEAS antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 297 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis image was generated using the ascites version of the product.
-
IF using ab11755.
This image was generated using the ascites version of the product.
-
ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (21)
ab11755 has been referenced in 21 publications.
- Smalley JL et al. Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes. Front Mol Neurosci 13:563091 (2020). PubMed: 33192291
- Fedoryshchak RO et al. Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife 9:N/A (2020). PubMed: 32975518
- Kudryashev JA et al. An Activity-Based Nanosensor for Traumatic Brain Injury. ACS Sens 5:686-692 (2020). PubMed: 32100994
- Sato K et al. The Sustained Release of Tafluprost with a Drug Delivery System Prevents the Axonal Injury-induced Loss of Retinal Ganglion Cells in Rats. Curr Eye Res 45:1114-1123 (2020). PubMed: 31994948
- Akamine S et al. GNAO1 organizes the cytoskeletal remodeling and firing of developing neurons. FASEB J 34:16601-16621 (2020). PubMed: 33107105