Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)

Overview

  • Product name
    Alpha-Glucosidase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Saliva, Serum, Cell culture extracts, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Range
    0.1 mU/well - 10 mU/well
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.

    Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Alpha Glucosidase Assay Buffer WM 1 x 25ml
    Alpha Glucosidase Positive Control 1 vial
    Alpha Glucosidase Substrate Mix Amber 1 x 0.3ml
    p-Nitrophenol Standard (100 mM) 1 x 100µl
  • Research areas
  • Relevance
    a-Glucosidase breaks down a-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, a-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to a- glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
  • Cellular localization
    Lysosome. Lysosome membrane.
  • Alternative names
    • Acid Maltase
    • Aglucosidase Alfa
    • EC 3.2.1.20
    • Glucosidase, Alpha; Acid
    • LYAG
    • Lysosomal Alpha-Glucosidase
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab174093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate

  • Time course of Alpha-Glucosidase Activity in various samples

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Kinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset: Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.

Protocols

References

ab174093 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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