Anti-alpha Glucosidase II antibody (ab96757)

Overview

  • Product name
    Anti-alpha Glucosidase II antibody
    See all alpha Glucosidase II primary antibodies
  • Description
    Rabbit polyclonal to alpha Glucosidase II
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 97-261 of Human alpha Glucosidase II (NP_938149).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH-3T3 whole cell lysates; HeLa cells; SW480 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab96757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
  • Tissue specificity
    Detected in placenta.
  • Pathway
    Glycan metabolism; N-glycan metabolism.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 31 family.
  • Cellular localization
    Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha glucosidase II alpha subunit antibody
    • Alpha-glucosidase 2 antibody
    • G2AN antibody
    • GANAB antibody
    • GANAB_HUMAN antibody
    • Glu II antibody
    • Glucosidase alpha neutral AB antibody
    • Glucosidase II alpha antibody
    • Glucosidase II subunit alpha antibody
    • GluII antibody
    • KIAA0088 antibody
    • Neutral alpha glucosidase AB antibody
    • Neutral alpha glucosidase AB precursor antibody
    • Neutral alpha-glucosidase AB antibody
    see all

Images

  • Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 107 kDa
  • All lanes : Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : Molt-4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 107 kDa
  • Immunohistochemical analysis of alpha Glucosidase II in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.
  • Immunofluorescence analysis of alpha Glucosidase II in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).

References

ab96757 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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