The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF: 1/1000 and higher.
WB: 1/10000 and higher. Detects a band of approximately 66-68 kDa (the human protein runs at ~68 kDa, while rat, mouse and other species run at ~66 kDa). Minor bands at ~150 kDa are probably covalent dimers, and bands at ~50 kDa represent alpha internexin breakdown products. (Predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue specificityFound predominantly in adult CNS.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Post-translational modificationsO-glycosylated. Phosphorylated upon DNA damage, probably by ATM or ATR.