The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: 1/20.
WB: 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 55 kDa).
Is unsuitable for IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue specificityFound predominantly in adult CNS.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Post-translational modificationsO-glycosylated. Phosphorylated upon DNA damage, probably by ATM or ATR.