Overview

  • Product nameAnti-alpha Internexin antibody
    See all alpha Internexin primary antibodies
  • Description
    Chicken polyclonal to alpha Internexin
  • Specificityab72382 is specific for the ~66k alpha Internexin protein. In Westerm Blotting minor bands seen at ~150k are probably covalent dimers and bands at ~50k represent alpha Internexin breakdown products.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant rat alpha Internexin expressed in and purified from E. coli.

  • Positive control
    • Rat cerebral cortex lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/500.

Target

  • FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • Tissue specificityFound predominantly in adult CNS.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Anti-alpha Internexin antibody images

  • Anti-alpha Internexin antibody (ab72382) at 1/5000 dilution + rat cerebral cortex lysate

    Predicted band size : 56 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa (possible dimer).

References for Anti-alpha Internexin antibody (ab72382)

ab72382 has not yet been referenced specifically in any publications.

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