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Synthetic peptide corresponding to residues in the tail domain (Human)
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab40758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/250 - 1/500.|
|ICC/IF||1/250 - 1/500.|
ab40758 staining alpha Internexin in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.01% Triton X-100, blocked with 5% serum for 2 hours at 4°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/250) at 4°C for 10 hours. An undiluted Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.
ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.