Anti-alpha Internexin antibody [EPR1529] (ab108302)


  • Product nameAnti-alpha Internexin antibody [EPR1529]
    See all alpha Internexin primary antibodies
  • Description
    Rabbit monoclonal [EPR1529] to alpha Internexin
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues from the C-terminus of human alpha Internexin

  • Positive control
    • SH-SY5Y cell lysate, Y79 cell lysate, mouse brain lysate, rat brain lysate, human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab108302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionClass-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
    • Tissue specificityFound predominantly in adult CNS.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageExpressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • 66 kDa neurofilament protein antibody
      • AINX_HUMAN antibody
      • Alpha Inx antibody
      • Alpha-internexin antibody
      • Alpha-Inx antibody
      • INA antibody
      • Internexin neuronal intermediate filament protein alpha antibody
      • MGC12702 antibody
      • NEF 5 antibody
      • NEF5 antibody
      • Neurofilament 5 (66kD) antibody
      • Neurofilament 5 antibody
      • Neurofilament 66 antibody
      • Neurofilament 66 tax binding protein antibody
      • Neurofilament-66 antibody
      • NF 66 antibody
      • NF-66 antibody
      • NF66 antibody
      • TXBP 1 antibody
      • TXBP1 antibody
      see all

    Anti-alpha Internexin antibody [EPR1529] images

    • All lanes : Anti-alpha Internexin antibody [EPR1529] (ab108302) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : Y79 cell lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Performed under reducing conditions.

      Predicted band size : 55 kDa

      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • ab108302, at 1/250, staining alpha Internexin in human brain tissue by immunohistochemistry
    • ab108302 showing negative staining in Normal colon tissue.

    • ab108302 showing negative staining in Normal tonsil tissue.

    • ab108302 showing negative staining in Normal liver tissue.

    • ab108302 showing negative staining in Normal breast tissue.

    References for Anti-alpha Internexin antibody [EPR1529] (ab108302)

    ab108302 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 5 µg
    Gel Running Conditions Reduced Denaturing (10% separating gel)
    Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
    Specification Hippocampal homogenate
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Feb 12 2014