a-Ketoglutarate Dehydrogenase Activity Assay Kit (Colorimetric) (ab185440)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 100 µU
Overview
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Product name
a-Ketoglutarate Dehydrogenase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Semi-quantitative -
Sensitivity
< 100 µU -
Product overview
Abcam’s α-Ketoglutarate Dehydrogenase Activity Assay kit (Colorimetric) (ab185440) provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K678 Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit. K678-100 is the same size as the 100 test size of ab185440.
α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinylCoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer IX 1 x 25ml Developer Solution III 1 vial KGDH Positive Control 1 x 50µl KGDH Substrate Mix 1 vial NADH Standard I 1 vial -
Research areas
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Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
Sequence similarities
Belongs to the alpha-ketoglutarate dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- 2 oxoglutarate dehydrogenase
- 2 oxoglutarate dehydrogenase complex component E1
- 2 oxoglutarate dehydrogenase mitochondrial
see all
Associated products
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Related Products
Images
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This is example data only.
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α-Ketoglutarate Dehydrogenase activity in rat heart (75 µg) and liver lysates (100 µg). Assays were performed following the kit protocol. This is example data only.
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α-Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 µg), yeast mitochondria prepared from S. Cerevisiae(10 µg) and in rat liver lysate (100 µg). Assays were performed following the kit protocol. This is example data only.
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab185440 has been referenced in 13 publications.
- Lee I & Yang NC Using Taguchi Method to Determine the Optimum Conditions for Synthesizing Parapyruvate. Molecules 27:N/A (2022). PubMed: 35335234
- Kumstel S et al. Targeting pancreatic cancer with combinatorial treatment of CPI-613 and inhibitors of lactate metabolism. PLoS One 17:e0266601 (2022). PubMed: 35452495
- Gu Y et al. IDH1 mutation contributes to myeloid dysplasia in mice by disturbing heme biosynthesis and erythropoiesis. Blood 137:945-958 (2021). PubMed: 33254233
- Shin D et al. Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer. Redox Biol 30:101418 (2020). PubMed: 31931284
- Fuentes-Retamal S et al. Complex Mitochondrial Dysfunction Induced by TPP+-Gentisic Acid and Mitochondrial Translation Inhibition by Doxycycline Evokes Synergistic Lethality in Breast Cancer Cells. Cells 9:N/A (2020). PubMed: 32053908