Anti-alpha Lactalbumin antibody [F20.16] (ab20212)
Key features and details
- Mouse monoclonal [F20.16] to alpha Lactalbumin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgM
Overview
-
Product name
Anti-alpha Lactalbumin antibody [F20.16]
See all alpha Lactalbumin primary antibodies -
Description
Mouse monoclonal [F20.16] to alpha Lactalbumin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified Alpha Lactalbumin
-
General notes
This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
Purity
Purified IgM -
Purification notes
This antibody was purified using affinity chromatography. The sample was eluted using a commercially bought Neutral Elution Buffer and exchanged into PBS using a G25 column. -
Primary antibody notes
This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells. -
Clonality
Monoclonal -
Clone number
F20.16 -
Myeloma
x63-Ag8.653 -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. -
Tissue specificity
Mammary gland specific. Secreted in milk. -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3906 Human
- Omim: 149750 Human
- SwissProt: P00709 Human
- Unigene: 72938 Human
-
Alternative names
- a-LACTA antibody
- alfaLA antibody
- Alpha-lactalbumin antibody
see all
Images
-
ICC/IF image of ab20212 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab20212 has been referenced in 1 publication.
- Burchell J et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). PubMed: 3905581