Anti-alpha Lactalbumin antibody [F20.16] (ab20212)

Overview

  • Product name
    Anti-alpha Lactalbumin antibody [F20.16]
    See all alpha Lactalbumin primary antibodies
  • Description
    Mouse monoclonal [F20.16] to alpha Lactalbumin
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Alpha Lactalbumin

  • General notes


    This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Purified IgM
  • Purification notes
    This antibody was purified using affinity chromatography. The sample was eluted using a commercially bought Neutral Elution Buffer and exchanged into PBS using a G25 column.
  • Primary antibody notes
    This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • Clonality
    Monoclonal
  • Clone number
    F20.16
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa.
IHC-Fr Use at an assay dependent concentration.

Use withn acetone fixed tissues.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • a-LACTA antibody
    • alfaLA antibody
    • Alpha-lactalbumin antibody
    • Lactalbumin alpha antibody
    • Lactose synthase B protein antibody
    • LALBA antibody
    • LALBA_HUMAN antibody
    • Lysozyme-like protein 7 antibody
    • LYZL7 antibody
    • MGC138521 antibody
    • MGC138523 antibody
    see all

Anti-alpha Lactalbumin antibody [F20.16] images

  • ICC/IF image of ab20212 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-alpha Lactalbumin antibody [F20.16] (ab20212)

This product has been referenced in:
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »

See 1 Publication for this product

Product Wall

The histology was carried out on frozen sections (10ug) of lactating mammary glands.

It seems that lactating tissue is the most appropriate positive control, as normal and cancerous tissue does not appear to express alpha lactalbumin accordi...

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Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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