Overview

  • Product name
  • Description
    Rabbit polyclonal to alpha MSH
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic Human MSH alpha conjugated to Bovine thyroglobulin by a Glutaraldehyde linker (P01189).

  • Positive control
    • Rat pituitary gland tissue.
  • General notes
    For long-term storage, store lyophilized antibody until ready to use at -15º C or lower. After reconstitution, use immediately or refrigerate at 2º-8º C up to 2 days. For long-term storage, appropriately aliquot antibody to avoid repeated freeze/thaw cycles and freeze at -15° C or lower

Properties

Applications

Our Abpromise guarantee covers the use of ab123811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/4000 - 1/6000. in PBS/0.3% Triton X-100 using Bn/Av-HRP technique. Use 4% paraformaldehyde for Fixation and post-fixation.

Target

  • Relevance
    Melanocyte-stimulating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation. It increases the pigmentation of skin by increasing melanin production in melanocytes.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Alpha MSH antibody
    • Melanotropin alpha antibody
    • POMC antibody
    • proopiomelanocortin antibody

Images

  • ab123811, at 1/4000, staining MSH alpha in rat pituitary gland tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Schmid T  et al. Loss of NSCL-2 in gonadotropin releasing hormone neurons leads to reduction of pro-opiomelanocortin neurons in specific hypothalamic nuclei and causes visceral obesity. J Neurosci 33:10459-70 (2013). ICC/IF ; Mouse . Read more (PubMed: 23785158) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
Yes - 0,3% Triton X-100 in PBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 01 2014

We have not tested if this product will also detect POMC.

If you would like to know more about POMC and alpha MSH detection, please see the following publication link (Fig 2).

http://www.sciencedirect.com/science/article/pii/S0006...

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Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chicken brain cryosections)
Specification
Chicken brain cryosections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.1% Triton X-100
Blocking step
4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 08 2012

The customer is right and I was wrong. Sorry for the confusion.

DISCOUNT CODE: *******
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and testab123811 in Chicken. This code will give you: ...

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Thank you for contacting us.

The source of this antibody informed me that the immunogen is in the N-terminal region of the Human MSH and is unlikely to cross-react with Chicken. See attachment for more details about the homology between sequen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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