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Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Abreviews (4)Q&A (2)Specific References (5)

Overview

  • Product nameAnti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]See all Alpha Skeletal Muscle Actin primary antibodies ...
  • Description
    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • SpecificityIn Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).
  • Tested applicationsIHC-Fr, IHC-P, ICC, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Purified striated muscle actin from rabbit.

Properties

Applications

Our Abpromise guarantee covers the use of ab28052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr IHC-Fr: 1/50 - 1/100.
IHC-P IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC ICC: Use at an assay dependent dilution.
ICC/IF ICC/IF: Use at an assay dependent concentration.
WB WB: 1/1000. Predicted molecular weight: 42 kDa.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ACTA antibodyACTA1 antibodyACTC antibody
      ACTC1 antibodyActin alpha cardiac muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyAlpha actin 1 antibodyAlpha-actin-1 antibodyASMA antibodyCardiac muscle alpha actin 1 antibodyMPFD antibodySkeletal muscle alpha actin 1 antibody
    see all

Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] images

  • Immunocytochemistry/ Immunofluorescence - alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052)
    Immunocytochemistry/ Immunofluorescence - alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052)

    ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

  • Western blot - alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052)
    Western blot - alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052)
    All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

    Lane 1 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
    Lane 2 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
    Lane 3 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

    Secondary
    HRP polymer conjugated donkey anti-mouse IgG at 1/1000
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 1 second

References for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

This product has been referenced in:
  • Islas JF  et al. Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors. Proc Natl Acad Sci U S A 109:13016-21 (2012). Human . Read more (PubMed: 22826236) »
  • Roy S  et al. Fra-2 mediates oxygen-sensitive induction of transforming growth factor beta in cardiac fibroblasts. Cardiovasc Res 87:647-55 (2010). Read more (PubMed: 20427335) »

See all 5 Publications for this product

Publishing research using ab28052 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 6 of 6 results for Abreviews and Q&A

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Votes: Highest First

Hi We use a lot of your NBR1 antibody listed below. I was wondering if there was a possibility of ordering a larger size? perhaps 100ug or 200ug instead of 50ug? If not, perhaps a quote if we order 3 lots in 1 go? BW  

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I ca...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 01 2011

I would like to ask you for help. We need a specific antibody only labeling cardiomyocytes. If possible, the antibody should be labeled already. I have already bought the TroponinI antibody (ab97711) of Abcam which is working quite well. If you could offe...

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Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha sarc...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Below Average
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Branchiostoma belcheri Tissue sections (whole-mount)
Specification whole-mount
Fixative Chilled 100% methanol or 4% paraformaldehyde/MOPS pH 7.5 overnight at 4°C, then stored in 75% Ethanol at -20°C
Antigen retrieval step None
Permeabilization Yes - 5% triton-X 100 / PBS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted May 10 2010

Western blot abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
Application Western blot
Sample Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
Loading amount 40 µg
Specification Neonatal Cardiac Myocytes
Treatment phenylephrine for 24 hours
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 02 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Neonatal Cardiac Myocytes)
Specification Neonatal Cardiac Myocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% NP40
Blocking step 2%BSA, 2% FBS in PBS with 0.1% NP40 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jun 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"