Anti-alpha sarcomeric Actin antibody [5C5], prediluted (ab1321)

Overview

  • Product nameAnti-alpha sarcomeric Actin antibody [5C5], prediluted
  • Description
    Mouse monoclonal [5C5] to alpha sarcomeric Actin, prediluted
  • SpecificityMonoclonal anti-alpha sarcomeric actin has been used as a marker for rhabdomyosarcoma. This antibody is specific to alpha skeletal and alpha cardiac muscle actins. It does not reacts with smooth muscle tissue.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Guinea pig, Cow, Human, Xenopus laevis, Carp, Snake
  • Immunogen

    BALB/C mice were injected with purified rabbit striated muscle actin.

Properties

Applications

Our Abpromise guarantee covers the use of ab1321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 18609095).
    IHC-P: Use at an assay dependent concentration.
    IHC-Fr: Use at an assay dependent concentration.
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.No further titration is required.

    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kDa. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region. There are different alpha isoforms specific for muscle tissues, i.e. skeletal muscle alpha, cardiac muscle alpha, and smooth muscle alpha, respectively.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • antibody
      • ACT antibody
      • ACTB antibody
      • actg 1 antibody
      • actg antibody
      • Actg1 antibody
      • Actin cytoplasmic 2 antibody
      • Actin gamma 1 antibody
      • Actin gamma 1 propeptide antibody
      • actl antibody
      • AL023024 antibody
      • Beta actin antibody
      • beta actin FE3 antibody
      • Cytoskeletal gamma actin antibody
      • DFNA20 antibody
      • DFNA26 antibody
      • E51 antibody
      • Gamma actin antibody
      • MGC117491 antibody
      • mgc28612 antibody
      see all

    Anti-alpha sarcomeric Actin antibody [5C5], prediluted images

    • ab1321 staining alpha sarcomeric Actin in formalin-fixed, paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry.

    References for Anti-alpha sarcomeric Actin antibody [5C5], prediluted (ab1321)

    This product has been referenced in:
    • Wang YC  et al. Oestrogen inhibits BMP4-induced BMP4 expression in cardiomyocytes: a potential mechanism of oestrogen-mediated protection against cardiac hypertrophy. Br J Pharmacol N/A:N/A (2014). Read more (PubMed: 25323043) »
    • Schimrosczyk K  et al. Liposome-mediated transfection with extract from neonatal rat cardiomyocytes induces transdifferentiation of human adipose-derived stem cells into cardiomyocytes. Scand J Clin Lab Invest 68:464-72 (2008). ICC/IF ; Human . Read more (PubMed: 18609095) »

    See all 2 Publications for this product

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    Thank you for contacting us.

    I have attached the protocols for IHC with paraffin and frozen sections as well as the ones for dual labelingusing the sequential and simultaneous IF staining method.

    Just in case here are the website l...

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    We can confirm that alpha cardiac striated muscle actin is also called sarcomeric actin. For example, Ab7799 reacts with alpha cardiac striated muscle (sarcomeric) and alpha skeletal muscle. Ab7817 reacts with alpha smooth muscle, does not react with...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"