Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)

Overview

  • Product nameAnti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control
    See all Alpha Skeletal Muscle Actin primary antibodies
  • Description
    Mouse monoclonal [337CT 30.10.1] to Alpha Skeletal Muscle Actin - Loading Control
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Pig, Orangutan
  • Immunogen

    Full length, recombinant human alpha sarcomeric Actin protein.

  • Positive control
    • This antibody gave a positive signal in HeLa and TE 671 whole cell lysates, and in the following tissue lysates: Human Heart (data not shown); Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab88226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block using 3% milk.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle antibody
    • Actin, alpha skeletal muscle antibody
    • ACTS_HUMAN antibody
    • Alpha actin 1 antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • Cardiac muscle alpha actin 1 antibody
    • MPFD antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control images

  • All lanes : Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226) at 5 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 6 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 30 seconds

References for Anti-Alpha Skeletal Muscle Actin antibody [337CT 30.10.1] - Loading Control (ab88226)

ab88226 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryo section)
Specification mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted May 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"