Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Overview

  • Product name
    Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
    See all Alpha Skeletal Muscle Actin primary antibodies
  • Description
    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • Specificity
    In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Purified striated muscle actin from rabbit.

Properties

Applications

Our Abpromise guarantee covers the use of ab28052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 42 kDa.

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle antibody
    • Actin, alpha skeletal muscle antibody
    • ACTS_HUMAN antibody
    • Alpha actin 1 antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • Cardiac muscle alpha actin 1 antibody
    • MPFD antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] images

  • ab28052 staining Alpha Skeletal Muscle Actin in rabbit heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 24 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

  • All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

    Lane 1 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
    Lane 2 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
    Lane 3 : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

    Secondary
    HRP polymer conjugated donkey anti-mouse IgG at 1/1000
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 1 secondThis image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

References for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

This product has been referenced in:
  • Bartlett JJ  et al. Doxorubicin impairs cardiomyocyte viability by suppressing transcription factor EB expression and disrupting autophagy. Biochem J 473:3769-3789 (2016). Read more (PubMed: 27487838) »
  • Moilanen AM  et al. WDR12, a Member of Nucleolar PeBoW-Complex, Is Up-Regulated in Failing Hearts and Causes Deterioration of Cardiac Function. PLoS One 10:e0124907 (2015). IF . Read more (PubMed: 25915632) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Rabbit Tissue sections (Heart)
Specification
Heart
Permeabilization
No
Fixative
Formalin 10%
Username

Dr. Nawazish Naqvi

Verified customer

Submitted Jul 14 2014

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 01 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Branchiostoma belcheri Tissue sections (whole-mount)
Specification
whole-mount
Fixative
Chilled 100% methanol or 4% paraformaldehyde/MOPS pH 7.5 overnight at 4°C, then stored in 75% Ethanol at -20°C
Antigen retrieval step
None
Permeabilization
Yes - 5% triton-X 100 / PBS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

金也 安井

Verified customer

Submitted May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
Loading amount
40 µg
Specification
Neonatal Cardiac Myocytes
Treatment
phenylephrine for 24 hours
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Richard Patten

Verified customer

Submitted Jun 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neonatal Cardiac Myocytes)
Specification
Neonatal Cardiac Myocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% NP40
Blocking step
2%BSA, 2% FBS in PBS with 0.1% NP40 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Richard Patten

Verified customer

Submitted Jun 02 2008

Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha s...

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