For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Purified striated muscle actin from rabbit.
Our Abpromise guarantee covers the use of ab28052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 42 kDa.|
ab28052 staining Alpha Skeletal Muscle Actin in rabbit heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 24 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.