You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)

Submit an Abreview Submit a Question

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab125057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        AAT6 antibodyACTA_HUMAN antibodyACTA2 antibody
        Actin alpha 2 smooth muscle aorta antibodyActin aortic smooth muscle antibodyActin, aortic smooth muscle antibodyACTSA antibodyACTVS antibodyAlpha 2 actin antibodyAlpha actin 2 antibodyAlpha cardiac actin antibodyAlpha-actin-2 antibodyCell growth inhibiting gene 46 protein antibodyCell growth-inhibiting gene 46 protein antibodyGIG46 antibodyGrowth inhibiting gene 46 antibodyMYMY5 antibody
      see all

    Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)
      IHC image of ab125057 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-alpha smooth muscle Actin antibody [1A4] (Biotin) (ab125057)

    ab125057 has not yet been referenced specifically in any publications.

    Publishing research using ab125057 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab125057 Submit a Question for ab125057

    There are currently no Abreviews or Questions for ab125057.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"