Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibody [1A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • SpecificityThis antibody reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It reacts with myofibroblasts induced by TGF-h1 treatment (see Cushing MC et al. 2005).
  • Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Human, Pig
    Predicted to work with: Cow, all Mammals, Baboon
  • Immunogen

    The N-terminal decapeptide of alpha-smooth muscle actin.

  • Positive control
    • Bowel
  • General notes

     

    This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary.

Properties

Applications

Our Abpromise guarantee covers the use of ab7817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 17699866
ELISA 1/200 - 1/1000.
IHC-P 1/50 - 1/75.
IHC-Fr Use at an assay dependent dilution.
WB 1/100 - 1/300.
ICC Use at an assay dependent dilution.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin 2 antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [1A4] images

  • ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

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  • ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hours at 25°C. ab96875, DyLight®488-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Costained with ab92547, Rabbit anti-Vimentin (red).

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  • ab7817 staining alpha smooth muscle Actin (red) in Human lung adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehye, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1x TBS) for 3 hours at 25°C. An Alexa Fluor®633-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with Hoeches 33258 (blue).

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  • ab7817 staining alpha smooth muscle Actin in mouse heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with TritonX-100 and blocked with 5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody 1/100 in blocking buffer for 2 hours. An Alexa Fluor® 488-conjugated Donkey monoclonal to mouse IgG, dilution 1/200, was used as secondary antibody.

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  • Cannabinoid receptor (CB1) (green) and α-smooth muscle actin immunoreactivity (red) in a cross-section of rat middle cerebral artery. Rat middle cerebral artery sections were incubated for 12 hours with a 1:300 dilution of anti-CB1 polyclonal antibody raised in rabbits and a 1:100 dilution of anti-α-smooth muscle actin monoclonal antibody raised in mice. The secondary antibodies were Alexa-488-labeled goat anti-rabbit at a 1:500 dilution and Cy3-labeled goat anti-mouse at a 1:1,000 dilution. This picture was submitted by David Rademacher whose review of the antibody is in the review section of this datasheet.

  • All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1/200 dilution

    Lane 1 : HeLa Nuclear
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Fluorescence detection of secondary antibody.
  • Ab7817 positively staining paraformaldehyde fixed porcine valvular interstitial cells (VICs) at 1/75. Staining was carried out in conjunction with goat anti mouse Alexa Ò488 (1/400).

    Ab 7817 was used to detect myofibroblsats among a heterogeneous interstitial cell type. The image shows heparin treated VICs labelled for alpha actin (green), and counterstained with propidium iodide (red).

    This image is an edited version of an image received courtesy of an Abreview on on 22 August 2005. We do not have any further information relating to this image.

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  • Immunohistochemical analysis of mouse aorta (A) or skin (B) tissue, staining alpha smooth muscle Actin with ab7817.

    Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1% serum for 45 minutes 21°C; antigen retrieval was by enzymatic method in 0.0001% Trypsin-CaCl. Samples were incubated with primary antibody (4 µg/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A biotin-conjugated horse anti-mouse polyclonal IgG (1/50) was used as the secondary antibody.

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References for Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

This product has been referenced in:
  • Zhu Y  et al. Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic reticulum stress-mediated apoptosis. Life Sci 94:37-44 (2014). WB ; Rat . Read more (PubMed: 24220678) »
  • Zhang SM  et al. Interferon regulatory factor 8 modulates phenotypic switching of smooth muscle cells by regulating the activity of myocardin. Mol Cell Biol 34:400-14 (2014). Mouse . Read more (PubMed: 24248596) »

See all 41 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Human Tissue sections (lung tissue)
Specification lung tissue
Permeabilization Yes - H2O2 in MeOH
Fixative Paraformaldehyde
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Submitted Aug 21 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (endothelial cell)
Specification endothelial cell
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jun 11 2014

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12.5%)
Sample Mouse Tissue lysate - whole (lung)
Specification lung
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Miss. Hyerim SHIN

Verified customer

Submitted Jun 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Treatment BY for 4 weeks
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample Mouse Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Formaldehyde
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Submitted May 05 2014

Application Western blot
Loading amount 45 µg
Gel Running Conditions Non-reduced Denaturing (12.5%)
Sample Human Cell lysate - whole cell (Primary fibroblasts)
Specification Primary fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (A549 treated with TGF-b1)
Specification A549 treated with TGF-b1
Permeabilization Yes - 0.1% Triton X-100 in PBS
Fixative Formaldehyde
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Submitted Nov 28 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Antigen retrieval step None
Sample Mouse Tissue sections (lung)
Specification lung
Permeabilization No
Fixative Paraformaldehyde
Username

Miss. Hyerim SHIN

Verified customer

Submitted Nov 27 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA - Buffer pH 9,0
Sample Human Tissue sections (Liver)
Specification Liver
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Nov 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (primary colon myofibroblasts)
Specification primary colon myofibroblasts
Permeabilization No
Fixative Acetone
Username

Dr. Charles Pallangyo

Verified customer

Submitted Jul 03 2013

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