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Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
Database link: P62736
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary.
Our Abpromise guarantee covers the use of ab7817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 17699866|
|ELISA||1/200 - 1/1000.|
|IHC-P||1/50 - 1/75.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/100 - 1/300.|
|ICC||Use at an assay dependent dilution.|
ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.
ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hours at 25°C. ab96875, a donkey anti-mouse DyLight®488 (1/1000) was used as the secondary antibody. Costained with ab92547, Rabbit anti-Vimentin (red).
ab7817 staining alpha smooth muscle Actin (red) in Human lung adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehye, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1x TBS) for 3 hours at 25°C. An Alexa Fluor®633-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with Hoeches 33258 (blue).
Cannabinoid receptor (CB1) (green) and
Ab7817 positively staining paraformaldehyde fixed porcine valvular interstitial cells (VICs) at 1/75. Staining was carried out in conjunction with goat anti mouse Alexa Ò488 (1/400).
Ab 7817 was used to detect myofibroblsats among a heterogeneous interstitial cell type. The image shows heparin treated VICs labelled for alpha actin (green), and counterstained with propidium iodide (red).
This image is an edited version of an image received courtesy of an Abreview on on 22 August 2005. We do not have any further information relating to this image.
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