Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17699866
ELISA 1/200 - 1/1000.
IHC-P 1/50 - 1/75.
IHC-Fr Use at an assay dependent concentration.
WB 1/100 - 1/300.
ICC Use at an assay dependent concentration.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin 2 antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [1A4] images

  • ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

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  • ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hours at 25°C. ab96875, a donkey anti-mouse DyLight®488 (1/1000) was used as the secondary antibody. Costained with ab92547, Rabbit anti-Vimentin (red).

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  • ab7817 staining alpha smooth muscle Actin (red) in Human lung adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehye, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1x TBS) for 3 hours at 25°C. An Alexa Fluor®633-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with Hoeches 33258 (blue).

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  • ab7817 staining alpha smooth muscle Actin in mouse heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with TritonX-100 and blocked with 5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody 1/100 in blocking buffer for 2 hours. An Alexa Fluor® 488-conjugated Donkey monoclonal to mouse IgG, dilution 1/200, was used as secondary antibody.

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  • Cannabinoid receptor (CB1) (green) and α-smooth muscle actin immunoreactivity (red) in a cross-section of rat middle cerebral artery. Rat middle cerebral artery sections were incubated for 12 hours with a 1:300 dilution of anti-CB1 polyclonal antibody raised in rabbits and a 1:100 dilution of anti-α-smooth muscle actin monoclonal antibody raised in mice. The secondary antibodies were Alexa-488-labeled goat anti-rabbit at a 1:500 dilution and Cy3-labeled goat anti-mouse at a 1:1,000 dilution. This picture was submitted by David Rademacher whose review of the antibody is in the review section of this datasheet.

  • All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1/200 dilution

    Lane 1 : HeLa Nuclear
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Fluorescence detection of secondary antibody.
  • Ab7817 positively staining paraformaldehyde fixed porcine valvular interstitial cells (VICs) at 1/75. Staining was carried out in conjunction with goat anti mouse Alexa Ò488 (1/400).

    Ab 7817 was used to detect myofibroblsats among a heterogeneous interstitial cell type. The image shows heparin treated VICs labelled for alpha actin (green), and counterstained with propidium iodide (red).

    This image is an edited version of an image received courtesy of an Abreview on on 22 August 2005. We do not have any further information relating to this image.

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  • Immunohistochemical analysis of mouse aorta (A) or skin (B) tissue, staining alpha smooth muscle Actin with ab7817.

    Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1% serum for 45 minutes 21°C; antigen retrieval was by enzymatic method in 0.0001% Trypsin-CaCl. Samples were incubated with primary antibody (4 µg/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A biotin-conjugated horse anti-mouse polyclonal IgG (1/50) was used as the secondary antibody.

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References for Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

This product has been referenced in:
  • Zhang SM  et al. Interferon regulatory factor 8 modulates phenotypic switching of smooth muscle cells by regulating the activity of myocardin. Mol Cell Biol 34:400-14 (2014). Mouse . Read more (PubMed: 24248596) »
  • Lee D  et al. Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury. PLoS One 9:e88417 (2014). Read more (PubMed: 24516656) »

See all 52 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (pig skin)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment burn with stem cells treatment
Specification pig skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted May 20 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Sample Human Cell (Human arterial myocytes)
Specification Human arterial myocytes
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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Submitted Apr 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step None
Sample Rhesus monkey Tissue sections (Renal artery (kidney))
Specification Renal artery (kidney)
Permeabilization No
Fixative 10% Neutral Buffered Formalin
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Submitted Apr 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako real target retrieval solution
Sample Rabbit Tissue sections (iliac)
Specification iliac
Permeabilization No
Fixative Formaldehyde
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Submitted Mar 04 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, HEK293)
Specification Hela, HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Feb 09 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Sample Mouse Cell (lung tissue)
Specification lung tissue
Permeabilization Yes - H2O2 in MeOH
Fixative Paraformaldehyde
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Submitted Jan 13 2015

Application Immunohistochemistry (Frozen sections)
Blocking step 1% BSA, 5% FBS in PBS as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Mouse Tissue sections (A375 tumor cells xenografted into Nude mice)
Specification A375 tumor cells xenografted into Nude mice
Permeabilization Yes - 0.3% Triton X-100
Fixative Formaldehyde
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Submitted Dec 16 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Rabbit Tissue sections (Ear)
Specification Ear
Permeabilization Yes - 0.05% tween 20
Fixative 10% formalin
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Submitted Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Pig Tissue sections (Skin Grafted wound)
Specification Skin Grafted wound
Permeabilization Yes - 0.05% tween 20
Fixative 10% formalin
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Submitted Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Human Tissue sections (chronic wound)
Specification chronic wound
Permeabilization Yes - 0.05% tween 20
Fixative 10% formalin
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Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"