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Anti-alpha smooth muscle Actin antibody [B4] (ab40863)

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Overview

Product name

Anti-alpha smooth muscle Actin antibody [B4]
See all alpha smooth muscle Actin products (20) ...

Description

Mouse monoclonal [B4] to alpha smooth muscle Actin

Specificity

Reacts selectively with enteric smooth muscle gamma-actin at high dilutions. It also can bind to alpha-actin present in skeletal, vascular and cardiac smooth muscle actin in preparations at low dilutions. It does not react with cytoplasmic beta- or gamma-actin.

Tested applications

IHC-P, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse

Predicted to work with

Chicken, all Mammals

Immunogen

Full length native protein (purified) (Chicken)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

B4

Isotype

IgG1

Light chain type

kappa

Research areas

Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Smooth Muscle Cells
Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin

  • Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody [B4] (ab40863)Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody [B4] (ab40863) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution.

  • ShowHide

    WB

     WB: 1/2000 - 1/500.Predi...Read more →

    WB: 1/2000 - 1/500.Predicted molecular weight: 42 kDa.

  • 1 Image

    ICC/IF

     ICC/IF: 1/100 - 1/500.

    ICC/IF: 1/100 - 1/500.

Target

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Involvement in disease

Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

Sequence similarities

Belongs to the actin family.

Cellular localization

Cytoplasm > cytoskeleton.

Target information above from: UniProt accessionP62736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AAT6 antibody
  • ACTA_HUMAN antibody
  • ACTA2 antibody
  • Actin alpha 2 smooth muscle aorta antibody
  • Actin aortic smooth muscle antibody
  • Actin, aortic smooth muscle antibody
  • ACTSA antibody
  • ACTVS antibody
  • Alpha 2 actin antibody
  • Alpha actin 2 antibody
  • Alpha cardiac actin antibody
  • Alpha-actin 2 antibody
  • Alpha-actin-2 antibody
  • Cell growth inhibiting gene 46 protein antibody
  • Cell growth-inhibiting gene 46 protein antibody
  • Growth inhibiting gene 46 antibody
see all

Database links

Anti-alpha smooth muscle Actin antibody [B4] images:

  Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody [B4] (ab40863)

Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody [B4] (ab40863)

Mouse detrusor muscle detected with ab40863 used at 1/500 fixed in paraformaldehye and embedded in paraffin.

References for Anti-alpha smooth muscle Actin antibody [B4] (ab40863)

This product has been referenced in:

  • Lessard JL Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton 10:349-62 (1988).Read more (PubMed: 2460261) »

See 1 publication for this product

Publishing research using ab40863? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"