alpha smooth muscle Actin antibody [B4] (ab40863)

Product Name 

alpha smooth muscle Actin antibody [B4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [B4] to alpha smooth muscle Actin

Immunogen 

Full length native protein (purified) (Chicken)

Reacts with 

Ms

Predicted to react 
(species key)

Mamm, Chk
(Due to sequence homology)

Specificity 

Reacts selectively with enteric smooth muscle gamma-actin at high dilutions. It also can bind to alpha-actin present in skeletal, vascular and cardiac smooth muscle actin in preparations at low dilutions. It does not react with cytoplasmic beta- or gamma-actin.

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100 - 1/500.
IHC-P: Use at an assay dependent dilution.
WB: 1/2000 - 1/500. Predicted molecular weight: 42 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin

Relevance 

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B4

Isotype 

IgG1

Light chain type 

kappa

Purity 

Ascites

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

5.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for alpha smooth muscle Actin antibody [B4] (ab40863)

  This clone has been used in:

Lessard JL Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton 10:349-62 (1988). PubMed: 2460261

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