![Western blot - alpha smooth muscle Actin antibody [E184] (ab32575) image](#ab32575_WB.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - alpha smooth muscle Actin antibody [E184] (ab32575) image](#ab32575_IHC.jpg)
![Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody [E184] (ab32575) image](#alpha-smooth-muscle-Actin-Primary-antibodies-ab32575-10.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Overview
Product name
Anti-alpha smooth muscle Actin antibody [E184]
See all alpha smooth muscle Actin products (20) ...
Description
Rabbit monoclonal [E184] to alpha smooth muscle Actin
Specificity
This antibody only detects actin in smooth muscle in immunohistochemistry.
Tested applications
IHC-Fr, WB, IHC-P, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide corresponding to N-terminus (Human)
Positive control
WB: A431 cell lysate IHC: uterus tissue
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 40% Glycerol, BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E184
Isotype
IgG
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Smooth Muscle Cells
Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/100
WB: 1/50000Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P: 1/500(Perform antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0.)
IP
IP: 1/20IP: 1/20
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similarities
Belongs to the actin family.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP62736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAT6 antibody
- ACTA_HUMAN antibody
- ACTA2 antibody
see all
Database links
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62738 Rat
- Unigene: 500483 Human
see all
Anti-alpha smooth muscle Actin antibody [E184] images:
Western blot - alpha smooth muscle Actin antibody [E184] (ab32575)
![Western blot - alpha smooth muscle Actin antibody [E184] (ab32575)](/ps/datasheet/Images/32/ab32575/ab32575_WB.jpg)
Anti-alpha smooth muscle Actin antibody [E184] (ab32575) at 1/50000 dilution + A431 cell lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
Immunohistochemistry (Paraffin-embedded sections) - alpha smooth muscle Actin antibody [E184] (ab32575)
![Immunohistochemistry (Paraffin-embedded sections) - alpha smooth muscle Actin antibody [E184] (ab32575)](/ps/datasheet/Images/32/ab32575/ab32575_IHC.jpg)
Immunohistochemical analysis of paraffin-embedded (A) human uterus tissue, showing positive staining of smooth muscle, and (B) skeletal muscle tissue, showing negative staining of skeletal muscle, using anti-alpha Actin (Smooth Muscle) (ab32572) diluted 1:500
Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody [E184] (ab32575)
![Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody [E184] (ab32575)](/ps/datasheet/images/32/ab32575/alpha-smooth-muscle-Actin-Primary-antibodies-ab32575-10.jpg)
ab32575 staining alpha smooth muscle actin in human kidney tumour sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 20 minutes at 21°C. Samples were incubated with primary antibody (1/100 in TBS + 2% BSA + 0.02% sodium azide) for 1 hour at 21°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-alpha smooth muscle Actin antibody [E184] (ab32575)
This product has been referenced in:
- Lendorf MEet al. Syndecan-1 and syndecan-4 are independent indicators in breast carcinoma. J Histochem Cytochem 59:615-29 (2011).Read more (PubMed: 21430259) »
- Engelhardt EMet al. A collagen-poly(lactic acid-co-?-caprolactone) hybrid scaffold for bladder tissue regeneration. Biomaterials 32:3969-76 (2011). IHC-P; Human.Read more (PubMed: 21377203) »
See all 9 publications for this product
Publishing research using ab32575? Please let us know so that we can cite the reference in this datasheet
![Anti-alpha smooth muscle Actin antibody [E184] for Immunohistochemistry (Frozen sections) in Human (32575)](/ps/datasheet/images/32/ab32575/alpha-smooth-muscle-Actin-Primary-antibodies-ab32575-8.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"