Anti-alpha smooth muscle Actin antibody (ab66133)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibodySee all alpha smooth muscle Actin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin
  • Tested applicationsWB, IP, Flow Cyt, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

    Does not react with

    Chicken
  • Immunogen

    A synthetic peptide (10aa) corresponding to N-terminal region alpha smooth muscle Actin.

  • Positive control
    • Mouse lung lysate, Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab66133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
IP Use a concentration of 2 - 5 µg/ml.
Flow Cyt Use 10-20µg for 106 cells.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin 2 antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody images

  • Human colon tissue stained with ab66133 at 1/200 dilution for 10 minutes at RT after antigen retrieval.

References for Anti-alpha smooth muscle Actin antibody (ab66133)

This product has been referenced in:
  • Chowdhury S  et al. Pirfenidone nanoparticles improve corneal wound healing and prevent scarring following alkali burn. PLoS One 8:e70528 (2013). WB . Read more (PubMed: 23940587) »
  • Bin WT  et al. Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action. World J Gastroenterol 18:309-22 (2012). IHC-P ; Rat . Read more (PubMed: 22294837) »

See all 3 Publications for this product

Product Wall

The main problem is the choice of secondary antibody. The one you are using, ab96977, is anti-rabbit IgA but the primary antibodies are rabbit IgG. You need an anti-rabbit IgG secondary. I recommend ab150088:

Click here (or use the following...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"