The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Human colon tissue stained with ab66133 at 1/200 dilution for 10 minutes at RT after antigen retrieval.
References for Anti-alpha smooth muscle Actin antibody (ab66133)
This product has been referenced in:
Mayfield AE et al. The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function. Biomaterials35:133-42 (2014).
Read more (PubMed: 24099706) »
Allahverdian S et al. Contribution of intimal smooth muscle cells to cholesterol accumulation and macrophage-like cells in human atherosclerosis. Circulation129:1551-9 (2014).
Read more (PubMed: 24481950) »