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Does not react withChicken
A synthetic peptide (10aa) corresponding to N-terminal region alpha smooth muscle Actin.
Our Abpromise guarantee covers the use of ab66133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|Flow Cyt||Use 10-20µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC-P||Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"