Anti-alpha smooth muscle Actin antibody (ab97375)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibodySee all alpha smooth muscle Actin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 134 - 366 of Human alpha smooth muscle Actin (NP_001604).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab97375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody images

  • All lanes : Anti-alpha smooth muscle Actin antibody (ab97375) at 1/10000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of Human fibroblasts, staining alpha smooth muscle Actin with ab97375.

    Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 16 hours at 4°C. Samples were incubated with primary antibody (1/1000 in diluent) for 16 hours at 4°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-alpha smooth muscle Actin antibody (ab97375)

ab97375 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Specification fibroblast
Fixative Paraformaldehyde
Permeabilization Yes - Triton-100
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

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Verified customer

Submitted Oct 22 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for your email.
We haven't tested this antibody in Zebrafish so we will not be able to comment on the specificity of this product. The antibody was tested in western blot with human cell line lysates and it gave good results which is why ...

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Thank you very much for your email.
The Zebrafish species is a predicted species for this antibody; this assumption is based on immunogen sequence homology only we unfortunately do not hold any tested data.
I have checked the expression of SMA ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"