Anti-alpha smooth muscle Actin antibody, prediluted (ab15267)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibody, predilutedSee all alpha smooth muscle Actin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin, prediluted
  • Tested applicationsIHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Baboon
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • Positive control
    • Smooth muscle or leiomyosarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab15267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
  • Application notesIHC-P: Ready to use for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
    IHC-Fr: Use at an assay dependent dilution (from PubMed:17149535).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAT6 antibody
      • ACTA_HUMAN antibody
      • ACTA2 antibody
      • Actin alpha 2 smooth muscle aorta antibody
      • Actin aortic smooth muscle antibody
      • Actin vascular smooth muscle antibody
      • Actin, aortic smooth muscle antibody
      • ACTSA antibody
      • ACTVS antibody
      • Alpha 2 actin antibody
      • Alpha actin 2 antibody
      • Alpha cardiac actin antibody
      • Alpha smooth muscle antibody
      • Alpha-actin 2 antibody
      • Alpha-actin-2 antibody
      • Cell growth inhibiting gene 46 protein antibody
      • Cell growth-inhibiting gene 46 protein antibody
      • GIG46 antibody
      • Growth inhibiting gene 46 antibody
      • MYMY5 antibody
      see all

    Anti-alpha smooth muscle Actin antibody, prediluted images

    • ab15267 staining alpha smooth muscle Actin in human leiomyoma by Immunohistochemistry (FFPE-sections).

    • ab15267 (used X1) staining day 3 wounded rat skin tissue by IHC-P. The tissue was formaldehyde fixed and blocked with peroxidase prior to incubation with the antibody for 25 minutes. An HRP conuugated goat polyclonal antibody was used as the secondary.

      See Abreview

    References for Anti-alpha smooth muscle Actin antibody, prediluted (ab15267)

    This product has been referenced in:
    • Evans DJ  et al. Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis. Circulation 119:116-22 (2009). IHC-P ; Mouse . Read more (PubMed: 19103996) »
    • Maclauchlan S  et al. Enhanced angiogenesis and reduced contraction in thrombospondin-2-null wounds is associated with increased levels of matrix metalloproteinases-2 and -9, and soluble VEGF. J Histochem Cytochem 57:301-13 (2009). Read more (PubMed: 19029404) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step solution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. R Berahovich

    Verified customer

    Submitted Aug 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cat Tissue sections (Skin)
    Specification Skin
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Jan 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Wound)
    Specification Wound
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Skin)
    Specification Skin
    Fixative Formaldehyde
    Antigen retrieval step None
    Blocking step peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 5
    Username

    Dr. Philip Roby

    Verified customer

    Submitted Nov 23 2006

    Thank you for your enquiry. For ab21027, cross-reactivity has not been specifically tested. By blast it looks like the only POSSIBLE problem would be cross-reactivity with gamma 2 actin but we wouldn't expect it. Ab15267 recognises alpha smooth m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"