Anti-alpha smooth muscle Actin antibody, prediluted (ab15267)

Overview

  • Product name
    Anti-alpha smooth muscle Actin antibody, prediluted
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin, prediluted
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Baboon
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • Positive control
    • Smooth muscle or leiomyosarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab15267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 17149535
IHC-P 1/1.

Ready to use for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.

Target

Images

  • ab15267 staining alpha smooth muscle Actin in human leiomyoma by Immunohistochemistry (FFPE-sections).

  • ab15267 (used X1) staining day 3 wounded rat skin tissue by IHC-P. The tissue was formaldehyde fixed and blocked with peroxidase prior to incubation with the antibody for 25 minutes. An HRP conuugated goat polyclonal antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Yang XR  et al. Upregulation of osmo-mechanosensitive TRPV4 channel facilitates chronic hypoxia-induced myogenic tone and pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 302:L555-68 (2012). Read more (PubMed: 22207590) »
  • Ren Y  et al. Overactivation of hedgehog signaling alters development of the ovarian vasculature in mice. Biol Reprod 86:174 (2012). Read more (PubMed: 22402963) »

See all 10 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
solution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. R Berahovich

Verified customer

Submitted Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Wound)
Specification
Wound
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 5
Username

Dr. Philip Roby

Verified customer

Submitted Nov 23 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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