The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
IHC-Fr: Use at an assay dependent dilution (from PubMed:17149535).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
ab15267 staining alpha smooth muscle Actin in human leiomyoma by Immunohistochemistry (FFPE-sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha smooth muscle Actin antibody, prediluted (ab15267)This image is courtesy of an Abreview submitted by Dr Philip Roby
ab15267 (used X1) staining day 3 wounded rat skin tissue by IHC-P. The tissue was formaldehyde fixed and blocked with peroxidase prior to incubation with the antibody for 25 minutes. An HRP conuugated goat polyclonal antibody was used as the secondary.
References for Anti-alpha smooth muscle Actin antibody, prediluted (ab15267)
This product has been referenced in:
Evans DJ et al. Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis. Circulation119:116-22 (2009).
Read more (PubMed: 19103996) »
Maclauchlan S et al. Enhanced angiogenesis and reduced contraction in thrombospondin-2-null wounds is associated with increased levels of matrix metalloproteinases-2 and -9, and soluble VEGF. J Histochem Cytochem57:301-13 (2009).
Read more (PubMed: 19029404) »