Anti-alpha smooth muscle Actin [1A4] antibody (ab7817)
- Product nameAnti-alpha smooth muscle Actin [1A4] antibodySee all alpha smooth muscle Actin primary antibodies ...
- DescriptionMouse monoclonal [1A4] to alpha smooth muscle Actin
- SpecificityThis antibody reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It reacts with myofibroblasts induced by TGF-h1 treatment (see Cushing MC et al. 2005).
- Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr, WB, ICC more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Human, Pig
Predicted to work with: Cow, all Mammals, Baboon
The N-terminal decapeptide of alpha-smooth muscle actin.
- Positive control
- General notes
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage buffer20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide
- PurityTissue culture supernatant
- Primary antibody notes This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary.
- Clonality Monoclonal
- Clone number1A4
- Light chain typekappa
Our Abpromise guarantee covers the use of ab7817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent dilution. PubMed: 17699866|
|ELISA||ELISA: 1/200 - 1/1000.|
|IHC-P||IHC-P: 1/50 - 1/75.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|WB||WB: 1/100 - 1/300.|
|ICC||ICC: Use at an assay dependent dilution.|
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
- Entrez Gene: 101021287 Baboon
- Entrez Gene: 515610 Cow
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 100009271 Rabbit
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62739 Cow
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62740 Rabbit
- SwissProt: P62738 Rat
- Unigene: 500483 Human
- Unigene: 213025 Mouse
- Unigene: 195319 Rat
- Unigene: 3114 Rat
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Anti-alpha smooth muscle Actin [1A4] antibody images
ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.
ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hours at 25°C. ab96875, DyLight®488-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Costained with ab92547, Rabbit anti-Vimentin (red).
ab7817 staining alpha smooth muscle Actin (red) in Human lung adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehye, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1x TBS) for 3 hours at 25°C. An Alexa Fluor®633-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with Hoeches 33258 (blue).
ab7817 staining alpha smooth muscle Actin in mouse heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with TritonX-100 and blocked with 5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody 1/100 in blocking buffer for 2 hours. An Alexa Fluor® 488-conjugated Donkey monoclonal to mouse IgG, dilution 1/200, was used as secondary antibody.
Cannabinoid receptor (CB1) (green) and
α-smooth muscle actin immunoreactivity (red) in a cross-section of rat middle cerebral artery. Rat middle cerebral artery sections were incubated for 12 hours with a 1:300 dilution of anti-CB1 polyclonal antibody raised in rabbits and a 1:100 dilution of anti- α-smooth muscle actin monoclonal antibody raised in mice. The secondary antibodies were Alexa-488-labeled goat anti-rabbit at a 1:500 dilution and Cy3-labeled goat anti-mouse at a 1:1,000 dilution. This picture was submitted by David Rademacher whose review of the antibody is in the review section of this datasheet.
All lanes : Anti-alpha smooth muscle Actin [1A4] antibody (ab7817) at 1/200 dilution
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Alexa Fluor anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Fluorescence detection of secondary antibody.
Ab7817 positively staining paraformaldehyde fixed porcine valvular interstitial cells (VICs) at 1/75. Staining was carried out in conjunction with goat anti mouse Alexa Ò488 (1/400).
Ab 7817 was used to detect myofibroblsats among a heterogeneous interstitial cell type. The image shows heparin treated VICs labelled for alpha actin (green), and counterstained with propidium iodide (red).
This image is an edited version of an image received courtesy of an Abreview on on 22 August 2005. We do not have any further information relating to this image.
Immunohistochemical analysis of mouse aorta (A) or skin (B) tissue, staining alpha smooth muscle Actin with ab7817.
Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1% serum for 45 minutes 21°C; antigen retrieval was by enzymatic method in 0.0001% Trypsin-CaCl. Samples were incubated with primary antibody (4 µg/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A biotin-conjugated horse anti-mouse polyclonal IgG (1/50) was used as the secondary antibody.
References for Anti-alpha smooth muscle Actin [1A4] antibody (ab7817)
This product has been referenced in:
- Raynaud CM et al. Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes. PLoS One 8:e54524 (2013). ICC/IF ; Human . Read more (PubMed: 23342164) »
- Barboni B et al. Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation. PLoS One 8:e63256 (2013). ICC/IF ; Sheep . Read more (PubMed: 23696804) »