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MCEEEDSTAL, corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin.
Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.
Our Abpromise guarantee covers the use of ab18147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
|IHC||Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18609095|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19338433|
ab18147 staining alpha smooth muscle actin in mouse muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An undiluted Phycoerythrin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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