Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibody [0.N.5]See all alpha smooth muscle Actin primary antibodies ...
  • Description
    Mouse monoclonal [0.N.5] to alpha smooth muscle Actin
  • SpecificityRecognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).
  • Tested applicationsIHC-P, ICC/IF, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Baboon
  • Immunogen

    Synthetic peptide: MCEEEDSTAL, corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin.

  • EpitopeAcetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
  • Positive control
    • Smooth muscle or leiomyosarcoma.
  • General notes

     

    Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ICC/IF
IHC-Fr
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 18609095).
    IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 19338433).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P62736 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AAT6 antibody
      • ACTA_HUMAN antibody
      • ACTA2 antibody
      • Actin alpha 2 smooth muscle aorta antibody
      • Actin aortic smooth muscle antibody
      • Actin, aortic smooth muscle antibody
      • ACTSA antibody
      • ACTVS antibody
      • Alpha 2 actin antibody
      • Alpha actin 2 antibody
      • Alpha cardiac actin antibody
      • Alpha-actin 2 antibody
      • Alpha-actin-2 antibody
      • Cell growth inhibiting gene 46 protein antibody
      • Cell growth-inhibiting gene 46 protein antibody
      • GIG46 antibody
      • Growth inhibiting gene 46 antibody
      • MYMY5 antibody
      see all

    Anti-alpha smooth muscle Actin antibody [0.N.5] images

    • Formalin-fixed, paraffin-embedded human leiomyoma stained with ab18147 at 1ug/ml at RT for 30 minutes using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.

    References for Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)

    This product has been referenced in:
    • Kim KH  et al. Matricellular protein CCN1 promotes regression of liver fibrosis through induction of cellular senescence in hepatic myofibroblasts. Mol Cell Biol 33:2078-90 (2013). Human . Read more (PubMed: 23508104) »
    • Tang JR  et al. Excess soluble vascular endothelial growth factor receptor-1 in amniotic fluid impairs lung growth in rats: linking preeclampsia with bronchopulmonary dysplasia. Am J Physiol Lung Cell Mol Physiol 302:L36-46 (2012). Read more (PubMed: 22003089) »

    See all 8 Publications for this product

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    Thank you for your enquiry and your interest in our products.

    I have checked the website and the datasheets of these three antibodies and it seems that only ab18147 has been tested for cross-reactivity with rabbit. The other two antibodies ar...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Rat lung tissue)
    Specification Rat lung tissue
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Ms. Jin-Seok Lee

    Verified customer

    Submitted Oct 14 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (esophagus)
    Specification esophagus
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Oct 01 2008

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Carotid artery)
    Specification Carotid artery
    Fixative Formaldehyde
    Antigen retrieval step None
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Username

    Dr. Caline Koh-Tan

    Verified customer

    Submitted Mar 21 2007

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (coronary artery)
    Specification coronary artery
    Antigen retrieval step Other
    Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"