Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)

Overview

  • Product nameAnti-alpha smooth muscle Actin antibody [0.N.5]
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Mouse monoclonal [0.N.5] to alpha smooth muscle Actin
  • SpecificityRecognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).
  • Tested applicationsIHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Baboon
  • Immunogen

    Synthetic peptide:

    MCEEEDSTAL

    , corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin.

  • EpitopeAcetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
  • Positive control
    • Smooth muscle or leiomyosarcoma.
  • General notes

     

    Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 18609095
IHC-Fr Use at an assay dependent concentration. PubMed: 19338433

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin 2 antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [0.N.5] images

  • ab18147 staining alpha smooth muscle actin in mouse muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An undiluted Phycoerythrin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Formalin-fixed, paraffin-embedded human leiomyoma stained with ab18147 at 1ug/ml at RT for 30 minutes using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.

References for Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)

This product has been referenced in:
  • Kumar S  et al. Smoking accelerates pancreatic cancer progression by promoting differentiation of MDSCs and inducing HB-EGF expression in macrophages. Oncogene N/A:N/A (2014). Read more (PubMed: 24909166) »
  • Kim KH  et al. Matricellular protein CCN1 promotes regression of liver fibrosis through induction of cellular senescence in hepatic myofibroblasts. Mol Cell Biol 33:2078-90 (2013). Human . Read more (PubMed: 23508104) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (muscle tissue)
Specification muscle tissue
Permeabilization Yes - 0.5%Triton X-100
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Apr 24 2014

Thank you for your enquiry and your interest in our products.

I have checked the website and the datasheets of these three antibodies and it seems that only ab18147 has been tested for cross-reactivity with rabbit. The other two antibodies ar...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat lung tissue)
Specification Rat lung tissue
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Jin-Seok Lee

Verified customer

Submitted Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (esophagus)
Specification esophagus
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Carotid artery)
Specification Carotid artery
Fixative Formaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Dr. Caline Koh-Tan

Verified customer

Submitted Mar 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (coronary artery)
Specification coronary artery
Antigen retrieval step Other
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"