Anti-alpha smooth muscle Actin antibody [1A4] (ab18147)

Overview

  • Product name
    Anti-alpha smooth muscle Actin antibody [1A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Specificity
    Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). The clone number has been updated from (0.N.5) to (1A4) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Baboon
  • Immunogen

    Synthetic peptide corresponding to alpha smooth muscle Actin aa 1-10 (N terminal).
    Sequence:

    MCEEEDSTAL

  • Epitope
    Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
  • Positive control
    • IHC-Fr: Mouse muscle tissue. WB: Human lung tissue lysate. NIH/3T3, SV40-LT and C1C12 whole cell lysate. IHC-P: Human leiomyoma tissue. ICC/IF: Rat MD-ASC cells.
  • General notes

     

    Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 18609095
IHC-Fr Use at an assay dependent concentration. PubMed: 19338433

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [1A4] images

  • ab18147 staining alpha smooth muscle actin in mouse muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An undiluted Phycoerythrin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab18147) at 1 µg/ml

    Lane 1 : Human lung tissue lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : SV40-LT whole cell lysate
    Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 10 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18147 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Formalin-fixed, paraffin-embedded human leiomyoma stained with ab18147 at 1 µg/ml at RT for 30 minutes using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.

  • MD-ASCs treated with and without E2 supplementation for 2 and 4 weeks. Scale bar = 100 μm.

    Rat MD-ASC (adipose-derived stem cells) were stained for alpha smooth muscle Actin (red) using ab18147 in ICC/IF.

References for Anti-alpha smooth muscle Actin antibody [1A4] (ab18147)

This product has been referenced in:
  • Scotti L  et al. Sphingosine-1-phosphate restores endothelial barrier integrity in ovarian hyperstimulation syndrome. Mol Hum Reprod N/A:N/A (2016). Human . Read more (PubMed: 27645281) »
  • Feng C  et al. Association of 17-ß Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering. PLoS One 11:e0164918 (2016). ICC/IF ; Rat . Read more (PubMed: 27783699) »

See all 15 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (muscle tissue)
Specification
muscle tissue
Permeabilization
Yes - 0.5%Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat lung tissue)
Specification
Rat lung tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Jin-Seok Lee

Verified customer

Submitted Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (esophagus)
Specification
esophagus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Carotid artery)
Specification
Carotid artery
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Dr. Caline Koh-Tan

Verified customer

Submitted Mar 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (coronary artery)
Specification
coronary artery
Antigen retrieval step
Other
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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