Mouse monoclonal [1A4] to alpha smooth muscle Actin
SpecificityRecognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). The clone number has been updated from (0.N.5) to (1A4) both clone numbers name the same antibody clone.
EpitopeAcetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
Smooth muscle or leiomyosarcoma.
Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Primary antibody notesStains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Use at an assay dependent concentration. PubMed: 18609095
Use at an assay dependent concentration. PubMed: 19338433
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Western blot - Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)
All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab18147) at 1 µg/ml
Lane 1 : Human Lung Tissue Lysate Lane 2 : NIH3T3 Whole Cell Lysate Lane 3 : SV40-LT Whole Cell Lysate Lane 4 : C2C12 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa Observed band size : 42 kDa
Exposure time : 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18147 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406
Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody [0.N.5] (ab18147)This image is courtesy of an anonymous Abreview
ab18147 staining alpha smooth muscle actin in mouse muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An undiluted Phycoerythrin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
Formalin-fixed, paraffin-embedded human leiomyoma stained with ab18147 at 1ug/ml at RT for 30 minutes using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.
References for Anti-alpha smooth muscle Actin antibody [1A4] (ab18147)
This product has been referenced in:
Scotti L et al. Sphingosine-1-phosphate restores endothelial barrier integrity in ovarian hyperstimulation syndrome. Mol Hum ReprodN/A:N/A (2016).
Read more (PubMed: 27645281) »
Feng C et al. Association of 17-ß Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering. PLoS One11:e0164918 (2016).
Read more (PubMed: 27783699) »