Anti-alpha smooth muscle Actin antibody [1A4] (FITC) (ab8211)




Our Abpromise guarantee covers the use of ab8211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 17222057

ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/50 - 1/75. (as well as on cell smears).
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 17237513Use at an assay dependent dilution (from Pubmed:17237513).


  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [1A4] (FITC) images

  • ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).

References for Anti-alpha smooth muscle Actin antibody [1A4] (FITC) (ab8211)

This product has been referenced in:
  • Gorchs L  et al. Cancer-associated fibroblasts from lung tumors maintain their immunosuppressive abilities after high-dose irradiation. Front Oncol 5:87 (2015). Flow Cyt ; Human . Read more (PubMed: 26029659) »
  • Yue H  et al. Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs. Exp Ther Med 10:1437-1444 (2015). IHC-P ; Pig . Read more (PubMed: 26622503) »

See all 22 Publications for this product

Product Wall

Thank you very much for your question. Indeed, the use of ab8211 in flow cytometry has been documented in the publication "Delayed Goblet Cell Hyperplasia, Acetylcholine Receptor Expression, and Worm Expulsion in SMC-Specific IL-4Rα–Deficient M...

Read More

Thank you for your email. Indeed, I can confirm that the protocol is in reference to ab8211. The use of blocking is to decrease unspecific signal and background. The Fc block with the monoclonal antibody 2.4G2 is to block the Fc receptors expre...

Read More

Thank you for contacting us. Unfortunately we do not have a permeabilizing kit for flow cytometry in our catalogue for the moment. This antibody should however work with the general classical permeabilization methods. If there should be a probl...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Aortic smooth muscle cells)
Specification Aortic smooth muscle cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X/methanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 29 2008

Thank you for your enquiry. The isotype listed on the data sheet is IgG2A. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Unfortunately this antiserum, alpha smooth muscle Actin ab8211 does not contain an IgG2a kappa isotype control. This antiserum is IgG sera. We do stock a mouse IgG2a, kappa isotype control (ab18454). I hope t...

Read More

Dear Angela As actin is highly conserved, I would expect ab8211 to work on rat tissue. I would be willing to say this with a strong degree of confidence. I am afraid that we don't have a polyclonal to alpha actin at the moment. I hope this helps.

Dear Susan This antibody should react with mouse a-smooth muscle actin. I suggest starting with a range of dilutions at 1:50, 1:100 and 1:500. This should give you a good idea of how to optimise it. Obviously the dilution required depends on a nu...

Read More

This antibody has not yet been tested for cross reactivity with chicken.