Anti-alpha smooth muscle Actin antibody (ab15734)

Overview

  • Product name
    Anti-alpha smooth muscle Actin antibody
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Rabbit polyclonal to alpha smooth muscle Actin
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human, Zebrafish
    Predicted to work with: Rabbit, Chicken, Cow
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human alpha smooth muscle Actin.

    (Peptide available as ab16102.)

  • Positive control
    • Hela Whole Cell Lysate A431 Whole Cell lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Kidney (Mouse) Tissue Lysate Ovary (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab15734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/200.
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Ab15734 detecting Alpha Smoth Muscle Actin in Rat Heart. The tissue were fixed (animal Perfused fixed) with 4% PFA and Later postfixed overnight i the same fixitive. The tissue is cryoprotected in 30% sucrose and cut uisng a cryostat

    See Abreview

  • All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Kidney (Mouse) Tissue Lysate
    Lane 5 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab15734 staining alpha smooth muscle Actin in Horse trachealis muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in ethanol prior to blocking with 10% BSA for 1 hour at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An EXPOSE Polyvalent HRP/DAB detection kit (ab80436) was used for detection.

    See Abreview

  • Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml + Recombinant Human alpha smooth muscle Actin protein (ab114148) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa


    Exposure time : 20 secondsAb15734 recognizes the full length tagged recombinant alpha small muscle Actin protein (ab114148) which has an expected molecular weight of 67 kDa.
  • All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa whole cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml
    Lane 4 : A431 cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat-anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa

References

This product has been referenced in:
  • Yang Q  et al. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol 3:484-499 (2017). IF ; Zebrafish . Read more (PubMed: 28462385) »
  • Li Y  et al. Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells. Stem Cell Res Ther 8:156 (2017). Read more (PubMed: 28676082) »

See all 16 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (peritoneal mesothelial cells)
Specification
peritoneal mesothelial cells
Permeabilization
No
Fixative
Acetone:methanol=1:1
Username

Abcam user community

Verified customer

Submitted Nov 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Mouse Tissue lysate - whole (Heart-day 0 and day 7 post MI LV)
Specification
Heart-day 0 and day 7 post MI LV
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Merry Lindsey

Verified customer

Submitted Sep 20 2013

The immunogen for product ab7817 is unique to this antibody and is not raised in alternative hosts. When reviewing the immunogen for ab15734 we saw not similarity with zebrafish and would not recommend this antibody for this species. We do offer an ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2012

In general, I would say that you should see a signal, however, I found that the developing state of the kidney and the localization of this protein is very important as well.

Please see the following publication and the attachment:

...

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Thank you for your patience. IgG ab27472 LOT: GR49002-1 I can confirm that the concentration is 2 ug/ml. Please do not hesitate to contact me with additional questions or concerns.

Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isoty...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Uterine smooth muscle (myometrium))
Loading amount
100 µg
Specification
Uterine smooth muscle (myometrium)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Feb 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal human prostate)
Specification
Normal human prostate
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Jan 20 2011

1-10 of 15 Abreviews or Q&A

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