The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody (ab15734)This image is courtesy of an Abreview submitted by Dr. Sophie Pezet
Ab15734 detecting Alpha Smoth Muscle Actin in Rat Heart. The tissue were fixed (animal Perfused fixed) with 4% PFA and Later postfixed overnight i the same fixitive. The tissue is cryoprotected in 30% sucrose and cut uisng a cryostat
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab15734)This image is courtesy of an anonymous Abreview.
ab15734 staining alpha smooth muscle Actin in Horse trachealis muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in ethanol prior to blocking with 10% BSA for 1 hour at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An EXPOSE Polyvalent HRP/DAB detection kit (ab80436) was used for detection.
Ab15734 recognizes the full length tagged recombinant alpha small muscle Actin protein (ab114148) which has an expected molecular weight of 67 kDa.
Western blot - Anti-alpha smooth muscle Actin antibody (ab15734)
All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : A431 cell lysate Lane 3 : HeLa whole cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml Lane 4 : A431 cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat-anti-Rabbit IgG at 1/10000 dilution
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Li Y et al. Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells. Stem Cell Res Ther8:156 (2017).
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