The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
Use at an assay dependent concentration.
Antibody detection limit dilution 1:4,000.
Use a concentration of 2 - 4 µg/ml.
Use at an assay dependent concentration. PubMed: 20947685
Permeabilisation with ~0.2% Triton (in PBS) is recommended
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)
Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + Human skeletal muscle lysate at 35 µg
Predicted band size : 42 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha smooth muscle Actin antibody (ab21027)Image courtesy of an anonymous Abreview.
ab21027 staining alpha smooth muscle Actin in mouse femoral artery by Immunohistochemistry (PFA perfusion fixed frozen sections.) Tissue was fixed in formaldehyde, permeabilized using 0.1% Triton, blocked using 10% serum for 1 hour at 22°C and then incubated with ab21027 at a 1/300 dilution for 16 hours at 4 °C. The secondary used was an Alexa Fluor®488 rabbit anti-goat used at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human (top) and mouse (bottom) lung tissue sections labelling alpha smooth muscle Actin with ab21027 at 2.5µg/ml. Image shows staining of the alveolar walls. Trypsin-mediated antigen retrieval, HRP-staining. Bar in right lower corner represents 50um.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab21027)Image courtesy of an anonymous Abreview.
ab21027 staining alpha smooth muscle Actin in mouse pancreas by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 3 hours at RT; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/100) for 16 hours. An Alexa Fluor® 647 donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody..
References for Anti-alpha smooth muscle Actin antibody (ab21027)
This product has been referenced in:
Monaghan K et al. Hyperglycemia and Diabetes Downregulate the Functional Expression of TRPV4 Channels in Retinal Microvascular Endothelium. PLoS One10:e0128359 (2015).
Read more (PubMed: 26047504) »
Inoue O et al. Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. PLoS One10:e0139357 (2015).
Read more (PubMed: 26418160) »