Overview

  • Product nameAnti-alpha smooth muscle Actin antibody
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Goat polyclonal to alpha smooth muscle Actin
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human alpha smooth muscle Actin aa 3-12 (N terminal).
    Sequence:

    EEEDSTALVC


    Database link: P62736

  • Positive control
    • Lysate prepared from human duodenum.

Properties

Applications

Our Abpromise guarantee covers the use of ab21027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

IHC-P Use a concentration of 2 - 4 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20947685

Permeabilisation with ~0.2% Triton (in PBS) is recommended

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody images

  • Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + Human skeletal muscle lysate at 35 µg

    Predicted band size : 42 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • ab21027 staining alpha smooth muscle Actin in mouse femoral artery by Immunohistochemistry (PFA perfusion fixed frozen sections.) Tissue was fixed in formaldehyde, permeabilized using 0.1% Triton, blocked using 10% serum for 1 hour at 22°C and then incubated with ab21027 at a 1/300 dilution for 16 hours at 4 °C. The secondary used was an Alexa Fluor®488 rabbit anti-goat used at a 1/1000 dilution.

    See Abreview

  • Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + human duodenum lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human (top) and mouse (bottom) lung tissue sections labelling alpha smooth muscle Actin with ab21027 at 2.5µg/ml. Image shows staining of the alveolar walls. Trypsin-mediated antigen retrieval, HRP-staining. Bar in right lower corner represents 50um.

  • ab21027 staining alpha smooth muscle Actin in mouse pancreas by Immunohistochemistry  (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 3  hours at RT; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/100) for 16 hours. An Alexa Fluor® 647 donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody..

    See Abreview

References for Anti-alpha smooth muscle Actin antibody (ab21027)

This product has been referenced in:
  • Monaghan K  et al. Hyperglycemia and Diabetes Downregulate the Functional Expression of TRPV4 Channels in Retinal Microvascular Endothelium. PLoS One 10:e0128359 (2015). Rat . Read more (PubMed: 26047504) »
  • Inoue O  et al. Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. PLoS One 10:e0139357 (2015). Read more (PubMed: 26418160) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (femoral artery)
Permeabilization Yes - 0.1% Triton-X
Specification femoral artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Dec 16 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MSC)
Permeabilization Yes - Triton 1%
Specification MSC
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
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Submitted Jul 08 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - Triton 1%
Fixative Paraformaldehyde
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Submitted Dec 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
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Submitted Nov 20 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Sample Human Tissue sections (Mammary fat pad)
Specification Mammary fat pad
Permeabilization No
Fixative Paraformaldehyde
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Submitted Apr 24 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample Mouse Tissue sections (Mammary fat pad)
Specification Mammary fat pad
Permeabilization No
Fixative Paraformaldehyde
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Submitted Apr 08 2014

Application IHC - Wholemount
Sample Mouse Tissue (Mammary fat pad)
Specification Mammary fat pad
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Submitted Apr 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization Yes - Triton-X
Fixative Acetone
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Submitted Apr 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (fibrotic mouse liver)
Loading amount 20 µg
Specification fibrotic mouse liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Submitted Nov 15 2012

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Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Heart)
Specification Heart
Fixative Acetone
Permeabilization Yes - 0.3 % Triton in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"