Anti-alpha Synuclein antibody [4D6] (ab1903)

Overview

  • Product nameAnti-alpha Synuclein antibody [4D6]
    See all alpha Synuclein primary antibodies
  • Description
    Mouse monoclonal [4D6] to alpha Synuclein
  • SpecificityThis antibody will not react with beta or gamma synuclein.
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-P, IHC-Fr, ELISAmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human alpha synuclein, produced in E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab1903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300. PubMed: 17227870Perfuse with 4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer and postfix.
WB 1/100 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IHC-Fr 1/100 - 1/1000.
ELISA Use at an assay dependent concentration.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similaritiesBelongs to the synuclein family.
    • DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      modifications
      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • PD 1 antibody
      • PD1 antibody
      • SNCA antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Anti-alpha Synuclein antibody [4D6] images

    • All lanes : Anti-alpha Synuclein antibody [4D6] (ab1903) at 5 µg/ml

      Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
      Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Additional bands at : 18 kDa (possible post-translational modification),80 kDa (possible post-translational modification).

    • IHC-P using ab1903 showing lewy bodies in a substantia nigra neuron(x400)

    References for Anti-alpha Synuclein antibody [4D6] (ab1903)

    This product has been referenced in:
    • Guo YJ  et al. Resveratrol alleviates MPTP-induced motor impairments and pathological changes by autophagic degradation of a-synuclein via SIRT1-deacetylated LC3. Mol Nutr Food Res 60:2161-2175 (2016). Read more (PubMed: 27296520) »
    • Wu F  et al. Rotenone impairs autophagic flux and lysosomal functions in Parkinson's disease. Neuroscience 284:900-11 (2015). Read more (PubMed: 25446361) »

    See all 18 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12)
    Sample Human Tissue lysate - whole (CNS tissue)
    Specification CNS tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Aug 01 2014

    Application Western blot
    Loading amount 75 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (Brain Tissue)
    Specification Brain Tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted May 13 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain)
    Loading amount 10 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing
    Blocking step odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Thank you for your email. We have not checked for specificity against the phosphorylated and non-phosphorylated form of alpha synuclein, therefore we do not have this information. If you do decide to test this antibody for specificity we ...

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    I'm sorry to hear your customer is having problems with ab1903. The customer's protocol seems to be for paraffin sections, is this correct? If it is, then it seems there is a problem with the vial they received, or the secondary antibody does not work...

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    The epitopes that are recognized by the synuclein antibodies ab1904, ab1903, ab24717, and ab36615 have not been mapped. I am sorry we could not be of more help. If you have any other questions, please do not hesitate to contact us.

    Thank you for your enquiry. We guarantee that this antibody will work in IHC and cross-react with mouse alpha synuclein. We would recommend using this antibody as per our general IHC protocol (protocols for both frozen and formalin-fixed paraffin-embe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"