Anti-alpha Synuclein antibody [LB 509] (ab27766)

Overview

  • Product nameAnti-alpha Synuclein antibody [LB 509]
    See all alpha Synuclein primary antibodies
  • Description
    Mouse monoclonal [LB 509] to alpha Synuclein
  • Specificityab27766 recognises alpha synuclein. Recent western blot re-tests performed by our lab showed negative results in both mouse and rat. Therefore, we won't recommend this antibody in these species.
  • Tested applicationsSuitable for: IHC-FoFr, ELISA, WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Lewy bodies purified from patients suffering dementia with Lewy bodies

  • EpitopeAb27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha synuclein.
  • Positive control
    • WB: full-length, recombinant alpha-synuclein protein.
  • General notes

    Alternative versions available:
    Anti-alpha Synuclein antibody (Alexa Fluor® 488) [LB 509] (ab208114)

Properties

Applications

Our Abpromise guarantee covers the use of ab27766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/500.
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-Fr 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Do not perform antigen retrieval.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similaritiesBelongs to the synuclein family.
    • DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      modifications
      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • PD 1 antibody
      • PD1 antibody
      • SNCA antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Anti-alpha Synuclein antibody [LB 509] images

    • IHC-P image of Alpha Synuclein (ab27766) staining in human brain samples with Multiple Systems Atrophy. The sections were subjected to heat-mediated antigen retrieval with citrate buffer. In addition, some slides received a 15 minute pre-treatment with Formic Acid. Sections were incubated in 20% serum for 30 minutes at +18°C to block non-specific protein-protein interactions. The sections were then incubated with ab27766 (1:400) for one hour at +18°C, followed by Biotin conjugated anti-mouse goat secondary antibody (1/200). Formic acid pre-treatment (15min) revealed more inclusions in MSA tissue.

      See Abreview


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 14 kDa


      Exposure time : 8 minutes
    • IHC-FoFr image of Alpha Synuclein staining on Synculein Transgenic mouse brain section stained using ab27766. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton 0.3X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody ab27766(1:500) overnight at +4°C. Mouse secondary was Alexa 488 and Granular ferritin expression (Alex 568) seen in the cytosol.

      See Abreview

    • Anti-alpha Synuclein antibody [LB 509] (ab27766) at 1/10000 dilution (Sample: ab51189,0.1ug)

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 14 kDa

    References for Anti-alpha Synuclein antibody [LB 509] (ab27766)

    This product has been referenced in:
    • Chai YJ  et al. Munc18-1 is a molecular chaperone for a-synuclein, controlling its self-replicating aggregation. J Cell Biol 214:705-18 (2016). Read more (PubMed: 27597756) »
    • Flores-Cuadrado A  et al. a-Synuclein staging in the amygdala of a Parkinson's disease model: cell types involved. Eur J Neurosci 41:137-46 (2015). IHC ; Mouse . Read more (PubMed: 25345880) »

    See all 11 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (SH-SY5Y)
    Specification SH-SY5Y
    Treatment 50 ´M, 100 ´M or 200 ´M of dopamine with or without 100 nM of MG132 proteasome inhibitor for 72 h
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. B Roberts

    Verified customer

    Submitted Feb 09 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Human Cell (SH-SY5Y)
    Specification SH-SY5Y
    Permeabilization Yes - 0.5% Tween
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (neuroblastoma)
    Permeabilization No
    Specification neuroblastoma
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 13 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing
    Sample Rat Tissue lysate - whole (brain tissue)
    Specification brain tissue
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 17 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Astrocytes)
    Permeabilization Yes - Methanol
    Specification Astrocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Permeabilization Yes - Methanol
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Neuroblastoma)
    Loading amount 15 µg
    Specification Neuroblastoma
    Gel Running Conditions Reduced Denaturing
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Thank you for contacting us. The reviewwer used 90-100% formic acid for 10 minutes. Please let me kknow if you have further questions.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab98929 and one vial of ab77028with the order number 1150942.
    <...

    Read More

    Thanks for sending the updated blot.

    I believe its expected that Synuclein should be detected in the rat brain extracts, which it is not. There may be a band in your overexpressed Synuclein protein but it is difficult to determine with the la...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"