Overview

  • Product nameAnti-alpha Tubulin antibodySee all alpha Tubulin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha Tubulin
  • Tested applicationsICC, ICC/IF, IHC-Fr, WB, IHC-P, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Pig, Fruit fly (Drosophila melanogaster), Zebrafish
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
  • Immunogen

    Synthetic peptide: ALEKDYEEVGVDSVEGEGEEEGEEY conjugated to KLH, corresponding to C terminal amino acids 426-450 of Human alpha Tubulin.

  • Positive control
    • Lung tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17785506
IHC-Fr Use at an assay dependent concentration. PubMed: 21069788
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin ubiquitous antibody
    • Alpha-tubulin 1 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha ubiquitous chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin K alpha 1 antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Anti-alpha Tubulin antibody images



  • Predicted band size : 50 kDa

    Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.

    This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

     

References for Anti-alpha Tubulin antibody (ab15246)

This product has been referenced in:
  • Friis TE  et al. A Polymerase Chain Reaction-Based Method for Isolating Clones from a Complimentary DNA Library in Sheep. Tissue Eng Part C Methods N/A:N/A (2014). WB ; Sheep . Read more (PubMed: 24447069) »
  • Mazel T  et al. Direct observation of microtubule pushing by cortical dynein in living cells. Mol Biol Cell 25:95-106 (2014). WB . Read more (PubMed: 24173713) »

See all 34 Publications for this product

Product Wall

Western blot

Excellent
Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (12.5)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted May 28 2013

Application Immunocytochemistry
Sample Human Cultured Cells (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 15°C
Username

Ms. AeLee Jeong

Verified customer

Submitted Apr 05 2013

Application Western blot
Sample Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Loading amount 30 µg
Specification High-fat diet-fed SD rat muscle
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

We would recommend the use of one of the following products for a loading control with zebrafish samples: AB15224 anti alpha Tubulin (http://www.abcam.com/alpha-tubulin-antibody-ab15246.html). This product has been featured in the following publicat...

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I am very pleased to hear you would like to accept our offer and test ab15246 in plasmodium falciparum. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for plasmodium falcipar...

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Thank you very much for your interest in ab15246.

To our knowledge, ab15246has not been tested in plasmodium falciparum. Therefore, I can offer a discount off a future purchase if you buy ab15246 now, test it in plasmodium falciparum and s...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293 T)
Total protein in input 100 µg
Specification HEK 293 T
Immuno-precipitation step Protein A/G
Username

Mr. Chris Tracy

Verified customer

Submitted Sep 18 2012

Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Loading amount 100000 cells
Specification Hela cells
Gel Running Conditions Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2012

Application Western blot
Sample Human Cell lysate - whole cell (HEK 293T)
Loading amount 10 µg
Specification HEK 293T
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Blocking buffer (LiCor, 927-40003) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 20 2012

Application Western blot
Sample Human Cell lysate - whole cell (Hek 293)
Loading amount 10 µg
Specification Hek 293
Gel Running Conditions Reduced Denaturing (10%)
Blocking step 1:1 PBS/Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Mr. Chris Tracy

Verified customer

Submitted Jan 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"