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Synthetic peptide: ALEKDYEEVGVDSVEGEGEEEGEEY conjugated to KLH, corresponding to C terminal amino acids 426-450 of Human alpha Tubulin.
Our Abpromise guarantee covers the use of ab15246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17785506|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21069788|
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.
This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"