Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)

Overview

  • Product nameAnti-alpha Tubulin antibody [B-5-1-2]See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [B-5-1-2] to alpha Tubulin
  • Tested applicationsWB, RIA, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Chlamydomonas reinhardtii, Sea Urchin
    Predicted to work with: Dog, Pig, Xenopus laevis, Cynomolgus Monkey, African Green Monkey, Xenopus tropicalis
  • Immunogen

    Full length protein corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

  • EpitopeThe antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.
  • Positive control
    • Total cell extract of the Human foreskin fibroblast cell line (FS11).
  • General notes

    Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 50 kDa.
RIA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF 1/100.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin ubiquitous antibody
    • Alpha-tubulin 1 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha ubiquitous chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin K alpha 1 antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Anti-alpha Tubulin antibody [B-5-1-2] images

  • All lanes : Anti-alpha Tubulin antibody [B-5-1-2] (ab11304) at 1/2000 dilution

    Lane 1 : Whole cell lysate prepared from HEK293 cells
    Lane 2 : Whole cell lysate prepared from HEK293 cells
    Lane 3 : Whole cell lysate prepared from HEK293 cells
    Lane 4 : Whole cell lysate prepared from HEK293 cells
    Lane 5 : Whole cell lysate prepared from HEK293 cells
    Lane 6 : Whole cell lysate prepared from HEK293 cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated mouse anti-rabbit at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    Image courtesy of an anonymous Abreview.

  • ab11304 staining  alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.

  • ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

     

    See Abreview

References for Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)

This product has been referenced in:
  • Khanna N  et al. MicroRNA-146b Promotes Myogenic Differentiation and Modulates Multiple Gene Targets in Muscle Cells. PLoS One 9:e100657 (2014). WB ; Mouse . Read more (PubMed: 24956113) »
  • Malek MH  et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). Mouse . Read more (PubMed: 23180811) »

See all 13 Publications for this product

Product Wall

Thank you for contacting us. I can confirm that the immungen used to generate this anti-alpha Tubulin antibody clone [B-5-1-2] was a preparation of sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin), as stated on the datashe...

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Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 30 µg
Specification HEK293
Treatment different transfection
Gel Running Conditions Reduced Denaturing (4-12% invitrogen bis-tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 27 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (C2c12)
Loading amount 30 µg
Specification C2c12
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 18 2010

Application Western blot
Sample Mouse Tissue lysate - whole (skeletal muscle)
Loading amount 50 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 10 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Methanol
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Submitted Apr 12 2006

Thank you for your email. The best way to search the neuronal markers that we carry is to do the following: under product browser (located on the left hand side of the Abcam homepage) click on Tags & Markers, then Cell Type Markers, then Neuroscien...

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