alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Product Name 

alpha Tubulin antibody [DM1A] - Loading Control

Product type 

Primary antibodies

Description 

Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control

Immunogen 

Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).

Reacts with 
(species key)

Hu, Ms, Rat, AGMk, Chk, Cow, Dog, Grb, Gpig, Hm, Pig, Xl

Specificity 

DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.

Epitope 

aa426-450

Tested applications 
(see key)

EM, ICC/IF, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Electron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml. FITC conjugated version availabe as ab64503.
IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining.
IP: Use at an assay dependent dilution.
WB: 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Lower background can be obtained by diluting ab7291 to 1:10000 and incubating overnight at 4C (Customer communication).

This antibody is ideal for use as a Western blot loading control.
Works under both reducing and non-reducing conditions.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Major constituent of Microtubules

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Isotype/Loading Controls >> Loading Controls >> Tubulin

Relevance 

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").

Excellent as a protein loading control antibody.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DM1A

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for alpha Tubulin antibody [DM1A] - Loading Control images, references, products related to ab7291 and other tools.

alpha Tubulin antibody [DM1A] - Loading Control images:

References for alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

  This product has been used in: (two most recent references)

Webber JL & Tooze SA Coordinated regulation of autophagy by p38alpha MAPK through mAtg9 and p38IP. EMBO J 29:27-40 (2010). WB; Human. PubMed: 19893488

Almad A & McTigue DM Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord. J Comp Neurol 518:785-99 (2010). WB; Rat. PubMed: 20058304

See all 53 publication references for this product.


If you publish research using ab7291 please let us know so that we can cite the reference on this datasheet.

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