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- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
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Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
(enlarge)


- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
(enlarge)


Product Name 

alpha Tubulin antibody [DM1A] - Loading Control

 

See all alpha Tubulin - Loading Control antibodies (5)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control

Immunogen 

Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).

Reacts with 
(species key)

Hu, Ms, Rat, AGMk, Chk, Cow, Dog, Grb, Gpig, Hm, Pig, Xl

Specificity 

DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.

Epitope 

aa426-450

Tested applications 
(see key)

EM, ICC/IF, IHC-Fr, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
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Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ICC/IF

16 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC-Fr

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• WB

27 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


WB
(read Abreview)


WB
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WB
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ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
Electron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml. FITC conjugated version availabe as ab64503.
IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining.
IP: Use at an assay dependent dilution.
WB: 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Lower background can be obtained by diluting ab7291 to 1:10000 and incubating overnight at 4C (Customer communication).

This antibody is ideal for use as a Western blot loading control.
Works under both reducing and non-reducing conditions.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Major constituent of Microtubules

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Isotype/Loading Controls >> Loading Controls >> Tubulin

Relevance 

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").

Excellent as a protein loading control antibody.

 

Alternative names alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

429035    (Chicken)
504244    (Cow)
7846    (Human)
7278    (Human)
7277    (Human)
22142    (Mouse)
64158    (Rat)
399313    (Xenopus laevis)

Omim

    

191110    (Human)

SwissProt

    

Q5E986    (Cow)
P68366    (Human)
Q71U36    (Human)
Q13748    (Human)
P68369    (Mouse)
P68370    (Rat)
Q4V850    (Xenopus laevis)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DM1A

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Tubulin antibody [DM1A] - Loading Control is on this datasheet. But please do contact us if you would like any reassurance!


See below for alpha Tubulin antibody [DM1A] - Loading Control images, references, products related to ab7291 and other tools.

 

alpha Tubulin antibody [DM1A] - Loading Control images:

Product image  - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Western blot using ab7291 on Hela Whole Cell Lysate (20 µg/lane).

Exposure: 10 sec.
Secondary: ab6728.

Lane 1: ab7291 at 1/5000
Lane 2: ab7291 at 1/10000

 

Product image  Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

ICC/IF image of ab7291 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab7291, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

 

Product image  - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

- alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Immunofluorescent imaging of human cells (U2OS) with ab7291 reveals a delicate network of alpha-tubulin (green) located exclusively in the cytoplasm. The nucleus is stained blue.

IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody Alexa 488 for 30 minutes.  All blocking and incubation steps carried out at 37 degrees.

Unfortunately, due to size constraints for images on our website, we are unable to show the full uncompressed picture in all its glory!

 

Product image  Western blot - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Western blot - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 0.5 µg/ml + Hela cell lysate

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) at 1/5000 dilution
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 50 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)


Exposure time : 30 seconds

 

Product image  Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

ab7291 at 1/400 staining MCF-10A cells (human mammary epithelial cell line) by ICC/IF. The cells were methanol fixed, blocked with BSA and incubated with the antibody for 16 hours. An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows alpha tubulin staining in green and DAPI counterstain in blue.

This image is courtesy of an anonymous Abreview

See Abreview

 

References for alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Specific publication references   This product has been used in: (two most recent references)

Webber JL & Tooze SA Coordinated regulation of autophagy by p38alpha MAPK through mAtg9 and p38IP. EMBO J 29:27-40 (2010). WB; Human. PubMed: 19893488

Almad A & McTigue DM Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord. J Comp Neurol 518:785-99 (2010). WB; Rat. PubMed: 20058304


See all 53 publication references for this product.


If you publish research using ab7291 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to alpha Tubulin

 

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alpha Tubulin antibody [DM1A] - Loading Control - related products:

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Loading controls

Lysates

 

Corresponding Antibody for alpha Tubulin antibody [DM1A] - Loading Control

ab64503

  

Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (FITC)

 

Compatible Secondaries for alpha Tubulin antibody [DM1A] - Loading Control

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

ab79091

  

Rat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chain

 

Other alpha Tubulin Antibodies

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alpha Tubulin antibody [DM1A] - Loading Control - other tools:

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