Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742)

Overview

  • Product nameAnti-alpha Tubulin antibody [DM1A] - Loading Control (HRP)See all alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control (HRP)
  • ConjugationHRP
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human, Xenopus laevis
  • Immunogen

    Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).

  • Positive control
    • This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab40742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).

Target

  • RelevanceMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
  • Cellular localizationMajor constituent of Microtubules
  • Database links
  • Alternative names
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 ALPHA antibody
    • Testis specific alpha tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • TUBA2 antibody
    • TUBA3 antibody
    • TUBA3C antibody
    • TUBA4A antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin, alpha 1a antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    see all

Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) images

  • All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

References for Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742)

This product has been referenced in:
  • Patoine D  et al. Decreased CYP3A expression and activity in guinea pig models of diet-induced metabolic syndrome: is fatty liver infiltration involved? Drug Metab Dispos 41:952-7 (2013). Guinea pig . Read more (PubMed: 23412133) »
  • Cameron RT  et al. Chemical informatics uncovers a new role for moexipril as a novel inhibitor of cAMP phosphodiesterase-4 (PDE4). Biochem Pharmacol 85:1297-305 (2013). WB . Read more (PubMed: 23473803) »

See all 6 Publications for this product

Product Wall

Thank you and your customer for taking the time to contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody after freezing.
The details your customer has kindly provided will provide us with...

Read More
Application Western blot
Sample Rat Cell lysate - whole cell (Neonatal Rat cardiomyocytes)
Loading amount 20 µg
Specification Neonatal Rat cardiomyocytes
Gel Running Conditions Reduced Non-Denaturing (Native) (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Mar 30 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 50 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (8% Tris/HCL)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"