Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC) (ab64503)

Overview

  • Product nameAnti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC)
    See all alpha Tubulin primary antibodies
  • Description
    Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAlpha Tubulin clone DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.
  • Tested applicationsIHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Human, Pig, Xenopus laevis, Gerbil
  • Immunogen

    Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).

  • Epitopeaa426-450. Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin.
  • Positive control
    • HeLa cells. Cultured human fibroblasts, baby hamster kidney (BHK) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
Flow Cyt Use 5.1µl for 106 cells.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
  • Cellular localizationMajor constituent of Microtubules
  • Database links
  • Alternative names
    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • H2 ALPHA antibody
    • Testis specific alpha tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • TUBA2 antibody
    • TUBA3 antibody
    • TUBA3C antibody
    • TUBA4A antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin, alpha 1a antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    see all

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC) images

  • ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab64503 staining alpha Tubulin in Xenopus laevis stage 36, transverse cryosection tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with MEMFa and blocked with 2% BSA/Serum for 1 hour at 230C. The sample was incubated with primary antibody (1/100) for 1 hour at 230C. No secondary antibody was used. FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI shows staining in grey. Laminin (Abcam`s ab11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary).

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC) (ab64503)

This product has been referenced in:
  • Li M  et al. Chromosomal aberrations in in-vitro matured oocytes influence implantation and ongoing pregnancy rates in a mouse model undergoing intracytoplasmic sperm injection. PLoS One 9:e103347 (2014). ICC/IF ; Mouse . Read more (PubMed: 25058692) »
  • Carone DM  et al. Hypermorphic expression of centromeric retroelement-encoded small RNAs impairs CENP-A loading. Chromosome Res 21:49-62 (2013). Read more (PubMed: 23392618) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Xenopus laevis Tissue sections (stage 36, transverse cryosection, Xenopus laevis)
Specification stage 36, transverse cryosection, Xenopus laevis
Fixative MEMFa
Permeabilization No
Blocking step BSA/Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
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Submitted Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"