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Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).
Our Abpromise guarantee covers the use of ab64503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 5.1µl for 106 cells. Ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab64503 staining alpha Tubulin in Xenopus laevis stage 36, transverse cryosection tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with MEMFa and blocked with 2% BSA/Serum for 1 hour at 230C. The sample was incubated with primary antibody (1/100) for 1 hour at 230C. No secondary antibody was used. FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI shows staining in grey. Laminin (Abcam`s ab11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary).
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