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Western blot - alpha Tubulin antibody [TU-01] (ab7750)
![Western blot - alpha Tubulin antibody [TU-01] (ab7750)](/ps/datasheet/images/ab7750_2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-alpha Tubulin antibody [TU-01](ab7750)
](\ps\datasheet\images\7\ab7750\alpha-Tubulin-Primary-antibodies-ab7750-1.jpg)
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Product Name
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alpha Tubulin antibody [TU-01]
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See all Tubulin (alpha) antibodies (20)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [TU-01] to alpha Tubulin
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Immunogen
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Fraction of tubulin purified from pig brain by two cycles of polymeriza tion-depol ymerizatio
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Reacts with
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Hu, Ms, At, Cow, Dog, Pig
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Predicted to react
(species key)
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Many, Mamm
(Due to sequence homology)
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Epitope
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aa 65-97 on N-terminal structural domain
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependant dilution.
ICC: 1/100. For ICC, we recommend not only fixing but also permeabilizing the cells.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IF: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution. Ab7749 (TU-16)is better for IP application.
WB: Use at an assay dependant dilution. Detects a band of approximately 55 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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TU-01
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites using precipitation methods.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Tubulin antibody [TU-01] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for alpha Tubulin antibody [TU-01] images, references, products related to ab7750 and other tools.
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alpha Tubulin antibody [TU-01] images:
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 Western blot - alpha Tubulin antibody [TU-01] (ab7750)
All lanes : alpha Tubulin antibody [TU-01] (ab7750) at 1/500 dilution
Lane 1 : Dog 1 heart whole tissue lysate (left ventricle)
Lane 2 : Dog 2 heart whole tissue lysate(left ventricle)
Lane 3 : Dog 3 heart whole tissue lysate (left ventricle)
Lane 4 : Dog 4 heart whole tissue lysate (left ventricle)
Lysates/proteins at 80 µg per lane.
Secondary
HRP conjugated sheep polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes This image is courtesy of an Abreview submitted by Dr sudhish mishra
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-alpha Tubulin antibody [TU-01](ab7750)
Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for alpha Tubulin antibody [TU-01] (ab7750)
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This product has been used in: (two most recent references)
Hemsley PA et al. Assaying protein palmitoylation in plants. Plant Methods 4:2 (2008). WB. PubMed: 18190696
Hale CM et al. Dysfunctional connections between the nucleus and the actin and microtubule networks in laminopathic models. Biophys J : (2008). Flow Cyt; Mouse. PubMed: 18790843
See all 11 publication references for this product.
If you publish research using ab7750 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to alpha Tubulin
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alpha Tubulin antibody [TU-01] - more information
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Customer reviews (feedback) regarding alpha Tubulin antibody [TU-01] |
Customer FAQs regarding alpha Tubulin antibody [TU-01] |
Protocols for alpha Tubulin antibody [TU-01] |
Price and availability of products related to alpha Tubulin antibody [TU-01] |
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alpha Tubulin antibody [TU-01] - related products:
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alpha Tubulin antibody [TU-01] - other tools:
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Contact Abcam with a Technical Enquiry about alpha Tubulin antibody [TU-01] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - alpha Tubulin antibody [TU-01] (ab7750)](/index.html?datasheet=7750#ab7750_2.jpg)
](/index.html?datasheet=7750#alpha-Tubulin-Primary-antibodies-ab7750-1.jpg)
