Anti-alpha Tubulin [B-5-1-2] antibody (ab11304)
- Product nameAnti-alpha Tubulin [B-5-1-2] antibodySee all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [B-5-1-2] to alpha Tubulin
- Tested applicationsWB, RIA, IP, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human, Chlamydomonas reinhardtii, African Green Monkey, Sea Urchin
Predicted to work with: Dog, Pig, Xenopus laevis, Cynomolgus Monkey, Xenopus tropicalis
Full length protein corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
- EpitopeThe antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.
- Positive control
- Total cell extract of the Human foreskin fibroblast cell line (FS11).
- General notes
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
- Concentration information loading...
- PurityProtein A purified
- Clonality Monoclonal
- Clone numberB-5-1-2
- Research Areas
Our Abpromise guarantee covers the use of ab11304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 50 kDa.|
|RIA||RIA: Use at an assay dependent dilution.|
|IP||IP: Use at an assay dependent dilution.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
- Entrez Gene: 418166 Chicken
- Entrez Gene: 777775 Cow
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Entrez Gene: 100038108 Xenopus tropicalis
- Omim: 191110 Human
- SwissProt: P09643 Chicken
- SwissProt: P81948 Cow
- SwissProt: P68367 Cynomolgus Monkey
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: P02550 Pig
- SwissProt: Q5XIF6 Rat
- SwissProt: P08537 Xenopus laevis
- SwissProt: Q28IX8 Xenopus tropicalis
- Unigene: 75318 Human
- Unigene: 1155 Mouse
- Unigene: 92961 Rat
- Alpha tubulin ubiquitous antibody
- Alpha-tubulin 1 antibody
- B ALPHA 1 antibody
- bA408E5.3 antibody
- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
- LIS3 antibody
- TBA4A_HUMAN antibody
- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
- Tuba4a antibody
- Tubulin alpha 1 chain antibody
- Tubulin alpha antibody
- Tubulin alpha ubiquitous chain antibody
- Tubulin alpha-1 chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin K alpha 1 antibody
- Tubulin, alpha 1 (testis specific) antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
- Tubulin, alpha-1 antibody
Anti-alpha Tubulin [B-5-1-2] antibody images
All lanes : Anti-alpha Tubulin [B-5-1-2] antibody (ab11304) at 1/2000 dilution
Lane 1 : Whole cell lysate prepared from HEK293 cells
Lane 2 : Whole cell lysate prepared from HEK293 cells
Lane 3 : Whole cell lysate prepared from HEK293 cells
Lane 4 : Whole cell lysate prepared from HEK293 cells
Lane 5 : Whole cell lysate prepared from HEK293 cells
Lane 6 : Whole cell lysate prepared from HEK293 cells
Lysates/proteins at 30 µg per lane.
HRP conjugated mouse anti-rabbit at 1/5000 dilution
developed using the ECL technique
Predicted band size : 50 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Image courtesy of an anonymous Abreview.
ab11304 staining alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.
ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
References for Anti-alpha Tubulin [B-5-1-2] antibody (ab11304)
This product has been referenced in:
- Malek MH et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). Mouse . Read more (PubMed: 23180811) »
- Chort A et al. Interferon beta induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice. Brain 136:1732-45 (2013). Read more (PubMed: 23518714) »