Anti-alpha Tubulin [DM1A] antibody (HRP) (ab40742)
- Product nameAnti-alpha Tubulin [DM1A] antibody (HRP)See all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [DM1A] to alpha Tubulin (HRP)
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Guinea pig, Human, Xenopus laevis
Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).
- Positive control
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate
- Storage instructionsStore at -20°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone numberDM1A
- Research Areas
Our Abpromise guarantee covers the use of ab40742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).|
- RelevanceMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
- Cellular localizationMajor constituent of Microtubules
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Entrez Gene: 399313 Xenopus laevis
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: Q13748 Human
- SwissProt: Q71U36 Human
- SwissProt: P68369 Mouse
- SwissProt: P68370 Rat
- SwissProt: Q4V850 Xenopus laevis
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Anti-alpha Tubulin [DM1A] antibody (HRP) images
All lanes : Anti-alpha Tubulin [DM1A] antibody (HRP) (ab40742) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
References for Anti-alpha Tubulin [DM1A] antibody (HRP) (ab40742)
This product has been referenced in:
- Patoine D et al. Decreased CYP3A expression and activity in guinea pig models of diet-induced metabolic syndrome: is fatty liver infiltration involved? Drug Metab Dispos 41:952-7 (2013). Guinea pig . Read more (PubMed: 23412133) »
- Cameron RT et al. Chemical informatics uncovers a new role for moexipril as a novel inhibitor of cAMP phosphodiesterase-4 (PDE4). Biochem Pharmacol 85:1297-305 (2013). WB . Read more (PubMed: 23473803) »