Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

Overview

  • Product name
    Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
    See all alpha Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
  • Specificity
    This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

  • Positive control
    • Jurkat cell lysates
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 54 kDa.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Sequence similarities
      Belongs to the tubulin family.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-tubulin 1 antibody
      • ALS22 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • MGC171407 antibody
      • MGC55332 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • tuba1l antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha-1 chain antibody
      • tubulin alpha-1B chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • tubulin, alpha 1, like antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] images

    • All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution

      Lane 1 : Jurkat cell lysates, untreated.
      Lane 2 : Jurkat cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    References for Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

    This product has been referenced in:
    • Paech F  et al. Hepatocellular Toxicity Associated with Tyrosine Kinase Inhibitors: Mitochondrial Damage and Inhibition of Glycolysis. Front Pharmacol 8:367 (2017). WB ; Human . Read more (PubMed: 28659801) »
    • Meshki J  et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . Read more (PubMed: 21966499) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab76290.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up