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A recombinant protein of Human alpha Tubulin.
Our Abpromise guarantee covers the use of ab125271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
ab125271 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"