Anti-alpha Tubulin [SPM359] antibody (ab125271)
- Product nameAnti-alpha Tubulin [SPM359] antibodySee all alpha Tubulin primary antibodies ...
- DescriptionMouse monoclonal [SPM359] to alpha Tubulin
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
A recombinant protein of Human alpha Tubulin.
- Positive control
- Human lung tissue; HeLa cells.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Constituent: 99% PBS
Note: contains antibody stabilizer
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberSPM359
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab125271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IP||IP: Use a concentration of 2 - 5 µg/ml.|
|IHC-P||IHC-P: 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
- Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
- Cellular localizationCytoplasm > cytoskeleton.
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Anti-alpha Tubulin [SPM359] antibody images
ab125271, at 1/100 dilution, staining alpha Tubulin in formalin-fixed Human lung tissue by Immunohsitochemistry.
References for Anti-alpha Tubulin [SPM359] antibody (ab125271)
ab125271 has not yet been referenced specifically in any publications.